微量凝集试验用大肠杆菌O50、O60、O117、O131定型血清的研制

doi:10.16431/j.cnki.1671-7236.2020.08.029

Abstract

Abstract: In order to improve and perfect the E.coli specific anti-O-antigen sera pool for micro-agglutination test in China,and further improve the preparation process of mono-specific anti-O-antigen serum,E.coli O50,O60,O117 and O131 serotypes were selected to investigate the method of preparing sera.First,inactivated antigens were prepared by reference strains of E.coli.Crude sera were extracted from rabbits after repeated immunizations by inactivated antigens.The method of micro-agglutination was used in the preparation process to determine the cross-lectins and agglutination value of different sera.The non-specific agglutination was eliminated by absorbing non-specific lectins and diluting the serum.Finally,E.coli specific anti-O-antigen sera for micro-agglutination test with good specificity were developed.The main cross-lectins of four kinds of E.coli specific anti-O-antigen sera were identified.Four kinds of sera with good specificity which could be used for E.coli O antigen micro-agglutination test were prepared,which agglutination titers were between 1:256 and 1:2 048.E.coli O50 and E.coli O60 serotypes sera were mono-specific anti-O-antigen sera.E.coli O117 and E.coli O131 serotypes sera contained 1-2 nonspecific cross-lectins,O14 and O107.This study was an important part of exploring the preparation process of mono-specific anti-O-antigen serum.The important theoretical bases were provided for improving the serum library of E.coli bacterial micro-agglutination test in China,and further improved the preparation method of E.coli O antigen serum for micro-agglutination test.

Key words: Escherichia coli; O antigen; micro-agglutination test; serum preparation

CLC Number:

S852.5⁺3

XIN Lingxiang, WANG Xiuli, ZHANG Yizhi, LI Jian, ZHANG Yuan. Development of E.coli O50,O60,O117,O131 Serotyped Sera for Micro-agglutination Test[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2596-2602.

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Development of E.coli O50,O60,O117,O131 Serotyped Sera for Micro-agglutination Test

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