大肠杆菌噬菌体BP16对鸡大肠杆菌病的防治效果

doi:10.16431/j.cnki.1671-7236.2022.02.040

Abstract

Abstract: 【Objective】 The study was aimed to investigate the effect of Escherichia coli phage BP16 on the prevention and treatment of chicken colibacillosis caused by O2 serotype avian pathogenic Escherichia coli (APEC) and the optimal therapeutic dose of phage BP16.【Method】 The fresh culture of O2 serotype APEC was diluted 5×10¹⁰, 5×10⁹, 5×10⁸, 5×10⁷ and 5×10⁶ CFU/mL 5 concentration gradients to determine the 50% lethal dose (LD₅₀) of APEC and determine its infection dose.13 kinds of commonly used drug disks with antibacterial or bactericidal effects on Gram-negative bacteria were selected for drug sensitivity test, and the positive control drugs were selected.The sterility and safety of phage lysate were determined by sterility test and safety test for subsequent tests.80 chicks were randomly divided into 5 experimental groups and 3 control groups.The experimental groups was intraperitoneally injected with Escherichia coli phage BP16 at different time before and after challenge, and the three control groups were intraperitoneally injected with florfenicol, Escherichia coli bacterial solution and normal saline respectively.The other conditions were the same.They were kept for 7 d, and the mortality of chicks was recorded, to evaluate the prevention and treatment effect of Escherichia coli phage BP16 on experimental chickens artificially infected with Escherichia coli.【Result】 The LD₅₀ of O2 serotype Escherichia coli was 1.5×10⁸ CFU/mL, florfenicol was selected as the positive control drug.The phage lysate was sterile and the phage suspension was safe for chicks and could be used in subsequent control tests.The use of phages in chickens 6 h before Escherichia coli infection could effectively prevent colibacillosis.The use of phage from the same time of infection to 6 h after infection could effectively treat colibacillosis, and the phage treatment effect was better than florfenicol.When the challenge dose of Escherichia coli was 1.5×10⁸ CFU, the phage treatment dose was 1.5×10⁹ PFU had the best therapeutic effect.【Conclusion】 Escherichia coli phage BP16 could prevent and treat colibacillosis.This study provided a scientific basis for the further application of phage to prevent and treat colibacillosis and the development of Escherichia coli phage preparation.

Key words: Escherichia coli phage; chicken colibacillosis; prevention and treatment

CLC Number:

S852.65

LI Min, ZHAO Zunfu, YANG Tingting, ZHANG Huanrong. Prevention and Treatment Effect of Escherichia coli Phage BP16 on Chicken Colibacillosis[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 776-782.

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Prevention and Treatment Effect of Escherichia coli Phage BP16 on Chicken Colibacillosis

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