China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (4): 1718-1728.doi: 10.16431/j.cnki.1671-7236.2023.04.042

• Basic Veterinary Medicine • Previous Articles    

Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis

CAO Yingying1,2,3, LIANG Liang1,2,3, ZENG Yirong1, ZHONG Qinghua1, YUAN Xiaofang1, TANG Haibo1,2,3, LENG Jing1,2,3   

  1. 1. Guangxi University of Chinese Medicine, Nanning 530200, China;
    2. Guangxi Key Laboratory of Translational Medicine for Treating High-incidence Infectious Diseases with Integrative Medicine, Nanning 530200, China;
    3. Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models of Guangxi, Nanning 530200, China
  • Published:2023-04-06

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Abstract: 【Objective】 The purpose of this study was to determine the cause of death of the Tupaia belangeri chinensis,and to find out the pathogenicity and drug resistance of isolated strain.【Method】 In this study,the pathogenic bacteria were isolated from the heart blood,liver,spleen,lung and kidney of diseased and dying Tupaia belangeri chinensis by the conventional plate scribing method.The strain was identified by morphological observation,biochemical test and identification of 16S rRNA gene.And drug sensitivity test and pathogenicity test were carried out.【Result】 A suspicious bacterial strain was isolated from the liver and lung of Tupaia belangeri chinensis,which died of illness.The isolate showed a single colony of light orange on McConkey medium,which was detected as Gram-negative short bacillus by microscopy.The biochemical test results showed that the isolated bacteria could decompose glucose,did not ferment lactose,produce gas,produce hydrogen sulfide,etc.,which conformed to the biochemical characteristics of Salmonella.Genetic evolution analysis showed that the isolate was closely related to the Salmonella CP082916 strain from broiler chickens,and the nucleotide similarity was 99%.The results of drug sensitivity test showed that the isolate was sensitive to chloramphenicol,gentamicin,neomycin,norfloxacin and other drugs,not sensitive to clindamycin and vancomycin.The results of pathogenicity test showed that the isolate had strong pathogenicity to Tupaia belangeri chinensis and adult mice,and the median lethal dose (LD50) to mice was 1.6×106 CFU.Histopathological observation showed that the alveolar septum of the lung of Tupaia belangeri chinensis and mice widened and inflammatory cells infiltrated in the septum.The arrangement of liver cells was disordered,with a large number of necrotic cells disintegrating,sinus space being deformed or reduced,blood stasis in the central vein,and inflammatory cells infiltrating around,some spleen cells were disordered.【Conclusion】 Salmonella was the key cause of the disease of the laboratory Tupaia belangeri chinensis and the isolated was sensitive to chloramphenicol,gentamicin and neomycin and had strong pathogenicity.

Key words: Tupaia belangeri chinensis; Salmonella; isolation and identification; 16S rRNA; drug resistance

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