华南虎子宫内膜炎摩氏摩根菌分离鉴定及生物学特性研究

doi:10.16431/j.cnki.1671-7236.2023.03.041

Abstract

Abstract: 【Objective】 The pathogen of a case of endometritis of an adult female South China tiger (Panthera tigris amoyensis) in Hangzhou Safari Park was isolated and identified,and its biological characteristics were studied.【Method】 The uterine secretions of the tiger were collected aseptically and the pathogenic bacteria were identified by using bacterial isolation and purification,morphological observation,biochemical identification of Merier Virtek^® 2 COMPACT system,and 16S rDNA sequence analysis.The pathogenicity of the clinical isolate was detected by intraperitoneal inoculation model of mice,and the sensitivity of the isolates to 50 drugs was identified by the universal Kirby-Bauer (K-B) test.The selected sensitive drugs were combined with symptomatic treatment to continuously observe whether the disease recurred and the birth situation.【Result】 A Gram-negative short rod-shaped bacillus isolate was obtained from the uterine secretion of the tiger.The identification result of Merrier Vitek^® 2 COMPACT system’s Gram negative card showed that the clinical isolated strain was Morganella morganii,which could ferment glucose,decompose mannose,D-glucose and urease,phosphatase,ornithine decarboxylase γ-glutamyltransferase reaction was positive.The sequence comparison analysis showed that the 16S rDNA sequence had the highest similarity with Morganella morganii,more than 99%.The results further showed that the bacterium was Morganella morganii,and was named HNH2019.The drug sensitivity results showed that the bacterium was multi resistant to 33 drugs,including polymyxin B,amikacin,cotrimoxazole,medimycin,tetracycline and cefoperazone,moderately sensitive to 6 drugs,including cefotaxime,ceftriaxone and cefoxitin,and sensitive to 11 drugs,including tobramycin,lomefloxacin,enoxacin and florfenicol.The results of mouse pathogenicity analysis showed that the median lethal dose of the isolate inoculated intraperitoneally in ICR mice was 1×10^7.5 CFU.After treatment with sensitive drugs,the tiger recovered and did not recur for three consecutive years.By September 2022,the female tiger had been pregnant many times and had given birth to five cubs in total.【Conclusion】 In this study,a Morganella morganii isolate was proposed as a potential pathogen of the South China tiger.After treatment with sensitive drugs selected by drug sensitivity test,the tiger recovered from endometritis without recurrence.The results of the study provided case reference and data support for the prevention and control of similar diseases,suggesting that attention should be paid to the potential harm of Morganella morganii to animals.

Key words: South China tiger (Panthera tigris amoyensis); Morganella morganii; endometritis; drug resistance

CLC Number:

S852.61⁺2

HU Yifan, MA Jinghua, BAI Siqi, ZHAI Yimeng, LIU Jianxun, SONG Houhui, YANG Yongchun. Isolation,Identification and Biological Characteristics Analysis of Morganella morganii from Endometritis of South China Tiger (Panthera tigris amoyensis)[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1271-1278.

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Isolation,Identification and Biological Characteristics Analysis of Morganella morganii from Endometritis of South China Tiger (Panthera tigris amoyensis)

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