地衣芽孢杆菌发酵条件优化和抑菌活性测定

doi:10.16431/j.cnki.1671-7236.2022.07.040

Abstract

Abstract: 【Objective】 The objective of this study was to investigate the effects of different culture and fermentation conditions on the antibacterial ability of Bacillus licheniformis 19148 and the resistance of Bacillus licheniformis 19148.【Method】 Bacillus licheniformis 19148 was cultured under different conditions of temperature,pH,metal ions,shaking speed,fermentation time and inoculation amount in a single factor experimental design,respectively,then centrifuge and filter the cultured liquid into sterile filtrate to study inhibition ability against Escherichia coli K88,Salmonella Typhimurium and Staphylococcus aureus 1882 using oxford cup bacteriostatic test.The drug resistance of Bacillus licheniformis 19148 to 27 antibiotics was studied by drug sensitive paper method.【Result】 Bacillus licheniformis 19148 could grow normally at 25,37 and 45 ℃, and was inhibited at 4 and 75 ℃,and had the best antibacterial activity at 37 ℃.Bacillus licheniformis 19148 could grow normally in the pH ranged from 5.0 to 9.0 and had the best antibacterial activity at pH 6.0.Bacillus licheniformis 19148 was intolerant to copper ions,but could tolerate magnesium ions,and there was no significant difference among treatment groups with different magnesium ion concentrations. In in vitro fermentation,Bacillus licheniformis 19148 showed the best antibacterial activity under the conditions of 3% seed liquid inoculation, and 150 r/min fermetation for 28 h.In the drug resistance evaluation test,Bacillus licheniformis 19148 showed moderate sensitivity to penicillin,tetracyclines,ceftazidine,furazolidone and polymyxin B,and showed high sensitivity to the other 22 antibiotics such as cefoperazone and erythromycin.【Conclusion】 Bacillus licheniformis 19148 had great stability and could be used in the production of industrial fermented feed without antibiotic resistance.

Key words: Bacillus licheniformis; fermentation conditions; antibacterial activity; drug resistance; probiotics

CLC Number:

S852.61^＋5

ZHANG Yu, MA Xi, ZHAO Jinbiao, LU Lin. Optimization of Fermentation Conditions and Determination of Antibacterial Activity of Bacillus licheniformis[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2812-2819.

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Optimization of Fermentation Conditions and Determination of Antibacterial Activity of Bacillus licheniformis

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