复合生物解毒剂在人工胃肠液中对黄曲霉毒素B1降解率的影响

doi:10.16431/j.cnki.1671-7236.2023.04.011

Abstract

Abstract: 【Objective】 In order to improve the degradation rate of aflatoxin B₁ (AFB₁) in animal feed,artificial gastrointestinal fluid (AGIF) was used to make compound biological detoxifier for degrading AFB₁ effectively.【Method】 Firstly,the degradation rates of AFB₁ by four probiotics in AGIF were investigated,then the optimal proportion of 4 species of beneficial microbes was obtained by means of response surface methodology to make compound probiotics for degrading AFB₁.And then the compound probiotics was combined with different concentrations of AFB₁-degrading enzyme to screen the optimal portfolio of compound biological detoxifier for degrading AFB₁ effectively.Finally,effect of compound biological detoxifier on degrading AFB₁ was evaluated under the AGIF condition.【Result】 ①The AFB₁-degradation rates by four species of Bacillus subtilis, Lactobacillus casein,Enterococcus faecalis and Candida utilis were 20.59%,19.97%,28.75% and 25.67%,respectively.② The maximal AFB₁-degradation rate was 41.5% when the visible counts of four probiotics were 1.0×10⁵,1.0×10⁵,1.0×10⁷ and 1.0×10⁵ CFU/mL to make compound probiotics by response surface methodology,significantly higher than that of single probiotic alone (P＜0.05);③When compound probiotics was combined with 0.1% AFB₁-degradation enzyme to make compound biological detoxifier,AFB₁-degradation rate was increased to 55.28%,significantly higher than that of probiotics or AFB₁-degradation enzyme alone (P＜0.05).【Conclusion】 The combination of compound probiotics with mycotoxin-degradation enzyme (compound biological detoxifier) was able to degrade AFB₁effectively.It could provide reference for biological detoxification measures of AFB₁ in feed.

Key words: aflatoxin B₁; compound probiotics; mycotoxin-degradation enzyme; artificial gastrointestinal fluid

CLC Number:

S816.7

GUO Hongwei, CAI Yongchao, FAN Chunguang, HAO Kaixuan, CHANG Juan, WANG Ping, LIU Chaoqi, YIN Qingqiang. Effect of Compound Biological Detoxifier on Aflatoxin B₁ Degradation Rate Under Artificial Gastrointestinal Fluid Condition[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1395-1403.

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Effect of Compound Biological Detoxifier on Aflatoxin B₁ Degradation Rate Under Artificial Gastrointestinal Fluid Condition

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Effect of Compound Biological Detoxifier on Aflatoxin B1 Degradation Rate Under Artificial Gastrointestinal Fluid Condition

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Effect of Compound Biological Detoxifier on Aflatoxin B₁ Degradation Rate Under Artificial Gastrointestinal Fluid Condition