黄曲霉毒素B1与M1对小鼠肠道细菌多样性的影响

doi:10.16431/j.cnki.1671-7236.2019.02.013

Abstract

Abstract:

To study the effect of aflatoxin B₁ and M₁ on intestinal bacterial diversity in mice,40 ICR male mice (4 weeks) were randomized into four groups of 10 each and received an oral administration of 0.3 mg/(kg·BW) AFB₁,3.0 mg/(kg·BW) AFM₁,mixture of AFB₁ and AFM₁ (0.3 mg/(kg·BW) AFB₁,3.0 mg/(kg·BW) AFM₁) and vehicle (1.0% DMSO) for 28 days (once a day at 9:00 am).Mice were executed after gavage,and intestinal contents were collected.The bacterial diversity of intestinal contents was analyzed by 16S rRNA sequencing.The results showed that compared with control group,there was no significant change in the dominant bacteria microflora in three experimental groups,regardless of the phylum level,family level or genus level(P>0.05).However,different toxin treatments still resulted in significant changes in the abundance of different bacteria microflora:Pathogenic bacteria or opportunistic pathogens abundance in the AFB₁ treatment and its combined treatment group with AFM₁,such as Facklamia,Staphylococcus and Corynebacterium were significantly increased compared with the control group (P<0.05),but not for AFM₁ group.Comprehensive analysis showed that individual AFB₁ or combined with AFM₁ could increase the abundance of pathogenic bacteria or opportunistic pathogens,and could change the healthy intestinal bacterial diversity,damage the function of intestinal microbial barrier.

Key words: aflatoxin B₁(AFB₁); aflatoxin M₁(AFM₁); bacterial diversity

CLC Number:

S859.87

ZHANG Muchen, ZHENG Nan, ZHAO Shengguo, ZHANG Yangdong, LI Songli, WANG Jiaqi. Effects of Aflatoxin B₁ and M₁ on Intestinal Bacterial Diversity in Mice[J]. , 2019, 46(2): 433-441.

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Effects of Aflatoxin B₁ and M₁ on Intestinal Bacterial Diversity in Mice

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Effects of Aflatoxin B1 and M1 on Intestinal Bacterial Diversity in Mice

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Effects of Aflatoxin B₁ and M₁ on Intestinal Bacterial Diversity in Mice