瘤胃保护性5-羟基色氨酸对绵羊肠道内容物褪黑素含量及菌群结构的影响

doi:10.16431/j.cnki.1671-7236.2019.03.007

Abstract

Abstract:

This study was aimed to investigate the effects of rumen protected 5-hydroxytryptophan (RPT-5-HTP) supplementation on melatonin content and microbial communities in gastrointestinal tract digesta of sheep,and explored the possibility of regulating sheep gastrointestinal tract microbial communities by RPT-5-HTP.Fifteen Kazak sheep which were 3 year old,average body weight (47.79±3.70) kg were chosen and divided into 3 groups:Control group,groups Ⅰ and Ⅱ.All sheep were fed with 12 g/(kg·BW) powder concentrate,1.8 kg corn silage every day,and had free continuous access to mixed hay.Additionally,each sheep in groups Ⅰ and Ⅱ was fed with RPT-5-HTP 111,222 mg/(kg·BW),respectively.The supplementary feeding experiment lasted for 25 d.At the end of the experiment,all sheep were slaughtered after the morning feeding (6 h),and the contents of jejunal,ileal,colonic and cecal digesta were taken to determine melatonin content and microflora structure.The results showed that the melatonin concentration in colonic digesta content of groups Ⅰ and jejunal and colonic digesta of groups Ⅱ were extremely significantly higher than that in control group (P<0.01),and that in ileal and cecal digesta of groups Ⅰ,Ⅱ were lower than control group,but there was no significant differences among three groups (P>0.05).The ACE and Chaol indexes of bacterial flora in the jejunal,cecal digesta of groups Ⅰ,Ⅱ were lower than those in control group (P>0.05).Compared with the control group,the bacterial diversity in cecal and colonic digesta were not significantly different at the levels of phylum and genus (P>0.05).The relative abundance of Firmicutes in the jejunal digesta was significantly increased in group Ⅰ (P<0.05),and the relative abundance of Tenericutes in group Ⅰ was significantly lower than that in control group (P<0.05).At the genus level,the relative abundance of Aeriscardovia in the jejunal digesta of group Ⅱ was increased (P<0.05).The relative abundance of Ruminococcaceae-NK4A214 in ileal digesta of groups Ⅰ,Ⅱ were significantly increased (P<0.05),while relative abundance of Clostridium-sensu-stricto and Romboutsia were significantly decreased (P<0.05).In conlusion,the RPT-5-HTP could increase melatonin concentration in the colonic digesta,but had no significant effect on the bacterial diversity of the gastrointestinal tract.

Key words: sheep; gastrointestinal tract; digesta; RPT-5-HTP; melatonin; bacterial diversity

CLC Number:

S823

ZHAO Fang, WANG Gen, ZHAO Guodong, GAO Chao, LI Xiaobin, MA Chen, YANG Kailun. Effect of Supplemented with Rumen-protected 5-hydroxytryptophan on Melatonin Content and Microbial Communities in Intestinal Tract Digesta of Sheep[J]. , 2019, 46(3): 690-702.

References

[1] 朱伟云,余凯凡,慕春龙,等.猪的肠道微生物与宿主营养代谢[J].动物营养学报,2014,26(10):3046-3051. ZHU W Y,YU K F,MU C L,et al.Gut microbiota and host nutrition metabolism in pigs[J].Chinese Journal of Animal Nutrition,2014,26(10):3046-3051.(in Chinese)
[2] BUBENIK G A,HACKER R R,BROWN G M,et al.Melatonin concentrations in the luminal fluid,mucosa,and muscularis of the bovine and porcine gastrointestinal tract[J].Journal of Pineal Research,1999,26(1):56-63.
[3] SWANSON G R,GORENZ A,SHAIKH M,et al.Decreased melatonin secretion is associated with increased intestinal permeability and marker of endotoxemia in alcoholics[J].American Journal of Physiology Gastrointestinal and Liver Physiology,2015,308(12):1004-1011.
[4] SOMMANSSON A,NYLANDER O,SJOBLOM M.Melatonin decreases duodenal epithelial paracellular permeability via a nicotinic receptor-dependent pathway in rats in vivo[J].Journal of Pineal Research,2013,54(3):282-291.
[5] PREZOTTO L D,LEMLEY C O,CAMACHO L E,et al.Effects of nutrient restriction and melatonin supplementation on maternal and foetal hepatic and small intestinal energy utilization[J].Journal of Animal Physiology and Animal Nutrition,2014,98(4):797-807.
[6] SOMMANSSON A,YAMSKOVA O,SCHIOTH H B,et al.Long-term oral melatonin administration reduces ethanol-induced increases in duodenal mucosal permeability and motility in rats[J].Acta Physiologica,2014,212(2):152-165.
[7] XU P,WANG J,HONG F,et al.Melatonin prevents obesity through modulation of gut microbiota in mice[J].Journal of Pineal Research,2017,62(4):1-13.
[8] REN W,WANG P,YAN J,et al.Melatonin alleviates weanling stress in mice:Involvement of intestinal microbiota[J].Journal of Pineal Research,2018,64(2):12448.
[9] ZHU D,MA Y,DING S,et al.Effects of melatonin on intestinal microbiota and oxidative stress in colitis mice[J].BioMed Research International,2018,2018:2607679.
[10] LEE P P,SHIU S Y,CHOW P H,et al.Regional and diurnal studies of melatonin binding sites in the duck gastrointestinal tract[J].Biological Signals,1995,4(4):212-224.
[11] HUETHER G.Melatonin synthesis in the gastrointestinal tract and the impact of nutritional factors on circulating melatonin[J].Annals of the New York Academy of Sciences,1994,719(1):146-158.
[12] LEE P P,PANG S F.Melatonin and its receptors in the gastrointestinal tract[J].Biological Signals,1993,2(4):181-193.
[13] NAMBOODIRI M A A,SUGDEN D,KLEIN D C,et al.5-hydroxytryptiphan elevates serum melatonin[J].Science,1983,221(4611):659-661.
[14] FERNÀNDEZ-CRUZ E,ÀLVAREZ-FERNÀNDEZ M A,VALERO E,et al.Validation of an analytical method to determine melatonin and compounds related to ltryptophan metabolism using UHPLC/HRMS[J].Food Analytical Methods,2016,9(12):1-10.
[15] BUBENIK G A,PANG S F,COCKSHUT J R,et al.Circadian variation of portal,arterial and venous blood levels of melatonin in pigs and its relationship to food intake and sleep[J].Journal of Pinal Research,2000,18(1):9-15.
[16] 赵芳,高超,王根,等.补喂5-羟基色氨酸、瘤胃保护性5-羟基色氨酸对绵羊血浆中5-羟色氨酸、褪黑素含量的影响[J].动物营养学报,2018,30(6):2143-2154. ZHAO F,GAO C,WAN G,et al.Effects of diet supplemented with 5-hydroxytryptophan,rumen protected 5-hydroxytryptophan on plasma 5-hydroxytryptophan and melatonin contents of sheep[J].Chinese Journal of Animal Nutrition,2018,30(6):2143-2154.(in Chinese)
[17] FERNANDEZ-GIL B,MONEIM A E,ORTIZ F,et al.Melatonin protects rats from radiotherapy-induced small intestine toxicity[J].PLoS One,2017,12(4):e0174474.
[18] HARDELAND R,FUHRBERG B.Ubiquitous melatonin-presence and effects in unicells,plants,and animals[J].Trends in Comparative Biochemistry & Physiology,1996,2:25-45.
[19] WANG J,FAN H,HAN Y,et al.Characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis[J].Asian-Australas Journal of Animal Science,2017,30(1):100-110.
[20] LEY R E,LOZUPONE C A,HAMADY M,et al.Worlds within worlds:Evolution of the vertebrate gut microbiota[J].Nature Reviews Microbiology,2008,6(10):776-788.
[21] CALLAWY T R,DOWD S E,EDRINGTON T S,et al.Evaluation of bacterial diversity in the rumen and feces of cattle fed different levels of dried distillers grains plus solubles using bacterial tag-encode FLX amplicon pyrosequencing[J].Journal of Animal Science,2010,88(12):3977-3983.
[22] MENEZES A B D,LEWIS E,DONOVAN M O,et al.Microbiome analysis of dairy cows fed pasture or total mixed ration diets[J].FEMS Microbiology Ecology,2011,78(2):256-265.
[23] MAO S,ZHANG M,LIU J,et al.Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle:Membership and potential function[J].Science Report,2015,5:16116-16129.
[24] LANGILLE M G,ZANEVELD J,CAPORASO J G,et al.Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences[J].Nature Biotechnology,2013,31(9):814-821.
[25] REIGSTAD C S,KASHYAP P C.Beyond phylotyping:Understanding the impact of gut microbiota on host biology[J].Neutogastroenterology and Motility,2013,25(2):358-372.
[26] JIAO J Z,WANG P,HE Z X,et al.In vitro evaluation on neutral detergent fiber and cellulose digestion by post-ruminal microorganisms in goats[J].Journal of the Science of Food and Agriculture,2013,94(9):1745-1752.
[27] 吴婷婷.补喂绵羊瘤胃液、益生菌对28日龄羔羊胃肠道菌群及免疫的影响[D].乌鲁木齐:新疆农业大学,2016. WU T T.The effects of supplement with ruminal fluid,probitotics on the gastrointestinal microbiota and immunity of lambs aged 28 days[D].Urumqi:Xinjiang Agricultural University,2016.(in Chinese)
[28] NISTAL E,CAMINERO A,HERRAN A R,et al.Differences of small intestinal bacteria populations in adults and children with/without celiac disease:Effect of age,gluten diet,and disease[J].Inflammatory Bowel Diseases,2012,18(4):649-656.
[29] GOORBACH S L,NAHAS L,LERNER P I,et al.Studies of intestinal microflora.1.Effects of diet,age,and periodic sampling on numbers of fecal microorganisms in man[J].Gastroenterology,1967,53(6):845-855.
[30] 谭碧娥,任文凯,印遇龙.胃肠道褪黑素分泌及其生理功能[J].动物营养学报,2018,30(4) 1207-1216. TAN B E,REN W K,YIN Y L.Secretion and physiological functions of melatonin in the gastrointestinal tract[J].Chinese Journal of Animal Nutrition,2018,30(4):1207-1216.(in Chinese)
[31] THEVARANJAN N,PUCHTA A,SCHULZ C,et al.Age-associated microbial dysbiosis promotes intestinal permeability,systemic inflammation,and macrophage dysfunction[J].Cell Host Microbe,2018,23(4):570.
[32] AKBARIAN A,KAZERANI H R,MOHRI M,et al.Exogenous melatonin improves growth performance,intestinal microbiota,and morphology in temporarily feed restricted broilers[J].Livestock Science,2014,167(1):400-407.
[33] 董晓丽.益生菌的筛选鉴定及其对断奶仔猪、犊牛生长和消化道微生物的影响[D].北京:中国农业科学院,2013. DONG X L.Identification of probiotics and effects of probiotics on weaned piglets,calves and the gastrointestinal microbiate[D].Beijing:Chinese Academy of Agricultural Sciences,2013.(in Chinese)
[34] SULLIVAN A,JOHANSSON A,SVENUNGSSON B,et al.Effect of lactobacillus f19 on the emergence of antibiotic resistant microorganisms in the intestinal microflora[J].The Journal of Antimicrobial Chemotherapy,2004,54(4):791-797.

Effect of Supplemented with Rumen-protected 5-hydroxytryptophan on Melatonin Content and Microbial Communities in Intestinal Tract Digesta of Sheep

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	LIU Jinyu, ZHANG Yang, MA Xumei, ZHOU Qian, ZHOU Yang, ZHANG Chunxiang, SHI Lei, REN Youshe. Effects of Glutathione on Cryopreservation of Sheep Semen [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2388-2394.
[3]	ZHANG Huaqiang, WANG Pinsheng, LI Xiao, WEI Panpeng, YAN Lingmin, JIANG Bingbing, WANG Zhaogui, WANG Xuefang, ZENG Huanqing, FAN Yi, GAO Zhenglong, WANG Xuebing. Effects of Fermented Hybrid Broussonetia papyrifera on Growth Performance, Meat Quality,Digestive Enzyme Activity and Intestinal Flora Structure of Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1876-1887.
[4]	ZHAO Qianming, QI Meng, XU Zeli, GONG Pihong, LU Chenyang, PEI Zhiyang, JIAN Fuchun, WANG Jiandong, JING Bo, NING Changsheng. Investigation of Intestinal Parasites Occurrence in Sheep and Environmental Contamination in Large-scale Housing Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2156-2165.
[5]	CUI Shuang, Wurlig, Alder, XIAO Min, WANG Jianqiu, GONG Jian. Effects of Yeast Selenium Supplementation on Gastrointestinal Microflora in Sheep Under Grazing Conditions [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 952-965.
[6]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[7]	ZHANG Mingliang, ZHANG Hailong, MA Shipeng, WANG Cailian, LANG Xia. Effects of Zanthoxylum bungeanum Essential Oil on Production Performance and Gastrointestinal Tissue Structure of Small-tail Han Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 556-563.
[8]	LI Chenglan, GUO Tingting, YUE Yaojing. Research Advances on Genomic Selection and Its Application in Sheep Breeding [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 616-625.
[9]	QIN Zhiyun, JIANG Huaizhi. An Overview of Research on Skin Hair Follicle Development and Regulatory Mechanism in Sheep During the Fetal Period [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 638-646.
[10]	AN Xiaopeng, LIU Nan, HU Zhengjiamei, HU Jincao, XU Xiaolong, ZHANG Lei, SONG Yuxuan. Comparative Analysis of Lactation Performance of East Friesen Sheep, Hu Sheep and East Friesen-Hu Hybrid Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 238-245.
[11]	LIU Gang, ZHU Fangxian, ZHANG Xiaowei, GU Feng, LIU Lijun, SU Rui, MENG Fei, XU Haitao, SUN Feizhou. Evaluation of Embryo and Sperm Cryopreservation in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3492-3499.
[12]	ZHANG Xueping, SHI Bingang, JIN Xiayang, WANG Xiangyan, LAN Lijuan, SHI Yu, QI Youpeng, ZHAO Shijie, LI Shaobin, HU Jiang. Expression Characterization Analysis of KLF7 Gene and Effects of Its Overexpression on Proliferation and Differentiation of Preadipocytes in Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2920-2930.
[13]	LI Weidong, JIANG Yuting, DUAN Hongwei, HE Haijun, YANG Shuai, DING Ziqiang, WU Jianxin, ZHANG Enqiu, HU Junjie. Distribution and Expression of Melatonin Receptors MT1 and MT2 in Various Stomach Tissues of Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3256-3262.
[14]	CAI Rui, ZHANG Shuai, CUI Xinyu, XU Chuncheng. Effect of Adding High Temperature Period Compost on Degradation of Organic Components and Enzyme Activity of Sheep Manure Composting on Qinghai Tibet Plateau [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2831-2841.
[15]	YAN Ani, SHENG Ben, WANG Hongrong, FENG Chunyan, WEI Zongyou. Effects of Rumen-protected Glucose and Slow-release Urea on Performance and Rumen Fermentation Function of Heat-Stressed Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2105-2115.