不同氨化处理黄曲霉毒素B1脱毒效果及其对奶牛瘤胃体外发酵的影响

doi:10.16431/j.cnki.1671-7236.2019.01.015

Abstract

Abstract:

This experiment was conducted to study the detoxification effect of amination on aflatoxin B₁ (AFB₁) by different ammonia treatments and its effect on the in vitro fermentation of dairy cows.This experiment was divided into two sections.Trial 1:The corn and rice mixture (1:3) artificially infected with 2 550 μg/kg AFB₁ were ammoniated,and they were randomly divided into control group (group Ⅰ,untreated) and test groups Ⅱ (0.1 Mpa,1 h),Ⅲ (0.1 Mpa,2 h),Ⅳ (0.1 Mpa,3 h),Ⅴ (0.2 Mpa,1 h),Ⅵ (0.2 Mpa,2 h),Ⅶ (0.2 Mpa,3 h),Ⅷ (0.3 Mpa,1 h),Ⅸ (0.3 Mpa,2 h),Ⅹ (0.3 Mpa,3 h) with 3 duplicates per group according to the difference in ammonia pressure and period.The detoxification effect of different ammonia treatments on AFB₁ and nutrient composition of the mixture were determined.Trial 2:The trail was divided into four treatments:Control group (group Ⅰ,untreated) and test groups Ⅱ (0.3 Mpa,1 h),Ⅲ (0.3 Mpa,2 h) and Ⅳ (0.3 Mpa,3 h) with 3 duplicates per group to explore the effect of ammoniated AFB₁ on the in vitro fermentation of dairy cows.The gas production,pH,in vivo dry matter degradation rate (IVDMD),ammonia nitrogen (NH₃-N) and volatile fatty acid (VFA) of the fermentation broth were measured after 2,4,8,12,24 and 48 h fermentation.The results showed that,in trail 1,the CP content of the test groups were significantly higher than that of control group (P < 0.05);The AFB₁ content of the group Ⅹ (0.3 Mpa,3 h) was significantly lower than that in the other groups (except group Ⅶ)(P < 0.05).In trail 2,the pH of fermentation broth of group Ⅳ (0.3 Mpa,3 h) was significantly lower than that of control group (P < 0.05),and the gas production,VFA,NH₃-N content and IVDMD (except 48 h) were significantly higher than that of control group (P < 0.05),while the effects of groups Ⅱ and Ⅲ were not obvious.In summary,ammonia treatment could significantly reduce the content of AFB₁,increase the CP content,and then improve the efficiency of ruminal fermentation,in which the treatment of 0.3 Mpa,3 h had the best effect.

Key words: aflatoxin B₁(AFB₁); ammonia; dairy cows; in vitro fermentation

CLC Number:

S816

ZHANG Meimei, JIANG Mengyu, SUN Youran, WEI Chuanzi, XIE Xiaolai. Effects of Different Ammonia Treatments on Aflatoxin B₁ Detoxification and Rumen Fermentation in Dairy Cows[J]. , 2019, 46(1): 130-139.

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Effects of Different Ammonia Treatments on Aflatoxin B₁ Detoxification and Rumen Fermentation in Dairy Cows

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Effects of Different Ammonia Treatments on Aflatoxin B1 Detoxification and Rumen Fermentation in Dairy Cows

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Effects of Different Ammonia Treatments on Aflatoxin B₁ Detoxification and Rumen Fermentation in Dairy Cows