新疆部分地区生乳中金黄色葡萄球菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2023.02.036

Abstract

Abstract: 【Objective】 The purpose of the experiment was to investigate the prevalence and drug resistance of Staphylococcus aureus in raw milk in some areas of Xinjiang,and provide a theoretical basis for monitoring Staphylococcus aureus in raw milk in this area and ensure the quality and safety of raw milk.【Method】 A total of 110 raw milk samples were randomly collected from large-scale dairy farms in Northern and Southern Xinjiang. Staphylococcus aureus was isolated and identified from milk samples by enrichment culture,isolation and purification,Gram staining,biochemical identification and PCR.The phenotypic resistance and drug resistance genes of Staphylococcus aureus to 16 antimicrobial agents were analyzed by broth microdilution and PCR.【Result】 18 strains of Staphylococcus aureus were isolated from 110 milk samples.The isolated strains showed light yellow,smooth and convex round colonies.Gram staining microscopy showed purple,short chain arrangement of Gram-positive bacteria.The biochemical test results conformed to the biochemical characteristics of Staphylococcus aureus.The isolated strains were identified as Staphylococcus aureus by 16S rDNA and nuc gene PCR amplification,with a separation rate of 16.36%(18/110).Drug susceptibility results showed that Staphylococcus aureus isolates had high resistance rates to ampicillin,penicillin and sulfaisoxazole,with resistance rates of 100%,83.33% and 77.78% respectively,while they were highly sensitive to vancomycin,compound minophen,oxacillin,cefalotin,ceftiofur,rifaximin and ciprofloxacin,with sensitivity rates of 100%,100%,94.44%,94.44%,94.44%, 94.44% and 94.44%,respectively.Among them,14 strains of Staphylococcus aureus were multidrug resistant,and the multidrug resistance rate was 77.78%.The detection results of drug resistance genes showed that the detection rate of macrolide resistance gene ermB (50.00%) was the highest,followed by β-lactam resistance gene mecA (27.28%) and Sul1 (22.22%).【Conclusion】 The prevalence and drug resistance rate of Staphylococcus aureus isolated from raw milk obtained from dairy farms in Xinjiang were still relatively serious,and the phenomenon of multi-drug resistance existed,which emphasized the importance of monitoring the drug resistance of Staphylococcus aureus in raw milk.

Key words: raw milk; Staphylococcus aureus; antibiotics; multiple drug resistance; drug resistance gene

CLC Number:

S852.61

MA Xiaojiao, ZHAO Yankun, SHAO Wei, WU Yating, LI Ming, LIU Huimin, MENG Lu, CHEN He. Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus in Raw Milk in Some Areas of Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 789-797.

References

[1] LIU Y,ZHENG X,XU L,et al.Prevalence,antimicrobial resistance,and molecular characterization of Staphylococcus aureus isolated from animals,meats,and market environments in Xinjiang,China[J]. Foodborne Pathogens and Disease,2021,18(10):718-726.
[2] Al-ASHMAWY M A,SALLAM K I,ABD-ELGHANY S M,et al.Prevalence,molecular characterization,and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolated from milk and dairy products[J]. Foodborne Pathogens and Disease,2016,13(3):156-162.
[3] 于忠娜,贾凌云,甄天元,等.新疆、内蒙古部分地区乳房炎奶样中金黄色葡萄球菌的流行性与耐药性分析[J].中国畜牧兽医,2019,46(4):1199-1208. YU Z N,JIA L Y,ZHEN T Y et al.Analysis of epidemicity and antimicrobial resistance of Staphylococcus aureus isolated from mastitis milk in Xinjiang and Inner Mongolia[J]. China Animal Husbandry & Veterinary Medicine,2019,46(4):1199-1208.(in Chinese)
[4] 苑晓萌,赵效南,李璐璐,等.山东地区乳房炎牛奶中大肠杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2021,48(1):312-323. YUAN X M,ZHAO X N,LI L L,et al.Isolation,identification and drug resistance analysis of E.coli from mastitis milk in Shandong province[J]. China Animal Husbandry & Veterinary Medicine,2021,48(1):312-323.(in Chinese)
[5] 蒋原.食源性病原微生物检测技术图谱[M].北京:科学出版社,2019. JIANG Y.Technical Atlas for Detection of Foodborne Pathogenic Microorganisms[M].Beijing:Science Press,2019.(in Chinese)
[6] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001. DONG X L,CAI M Y.Manual for Identification of Common Bacterial Systems[M].Beijing:Science Press,2001.(in Chinese)
[7] 田会丽,袁筠,廖超,等.石河子地区乳源性金黄色葡萄球菌毒力及耐药性的研究[J].现代食品科技,2017,33(9):265-270. TIAN H L,YUAN J,LIAO C,et al.Virulence characteristics and drug resistance of milk-derived Staphylococcus aureus isolates from the Shihezi region[J]. Modern Food Science and Technology,2017,33(9):265-270.(in Chinese)
[8] CLSI.Performance standards for antimicrobial susceptibility testing;Twenty-second informational supplement[S].Pennsylvania,USA:Clinical and Laboratory Standards Institute (CLSI).Document M100-S22.2018.
[9] 刘慧敏.我国奶业主产区生鲜乳中金黄色葡萄球菌耐药性及分子特征研究[D].北京:中国农业科学院,2018. LIU H M.Antimicrobial susceptibility,and molecular characterization of Staphylococcus aureus isolated from raw milk in China[D].Beijing:Chinese Academy of Agricultural Sciences,2018.(in Chinese)
[10] 杜伟伟.河北省乳源金黄色葡萄球菌耐药性检测及耐药基因的研究[D].保定:河北农业大学,2015. DU W W.Detection of drug resistance and study on drug resistance genes of Staphylococcus aureus from dairy sources in Hebei province[D].Baoding:Hebei Agricultural University,2015.(in Chinese)
[11] WANG X,MENG J,ZHOU T,et al.Antimicrobial susceptibility testing and genotypic characterization of Staphylococcus aureus from food and food animals[J]. Foodborne Pathogens and Disease,2012,9(2):95-101.
[12] ABDEEN E E,MOUSA W S,ABDEL SALAM S Y,et al.Antibiogram and phylogenetic diversity of enterotoxigenic Staphylococcus aureus strains from milk products and public health implications[J]. Saudi Journal of Biological Sciences,2020,27(8):1968-1974.
[13] SHITANDI A,STERNESJÖÅ.Prevalence of multidrug resistant Staphylococcus aureus in milk from large- and small-scale producers in Kenya[J]. Journal of Dairy Science,2004,87:4145-4149.
[14] 高延玲,董鹏,李金磊等.奶牛场金黄色葡萄球菌的分离及耐药性研究[J].畜牧与饲料科学,2014,35(9):4-5. GAO Y L,DONG P,LI J L,et al.Study on isolation and drug resistance of Staphylococcus aureus from dairy farm[J]. Animal Husbandry and Feed Science,2014,35(9):4-5.(in Chinese)
[15] 李光辉,郭卫芸,高雪丽,等.2003年~2015年金黄色葡萄球菌食物中毒事件特征分析[J].食品研究与开发,2018,39(6):200-203. LI G H,GUO W Y,GAO X L,et al.Epidemiological analysis of Staphyloccocus aureus food poisoning events in China during 2003-2015[J]. Food Research and Development,2018,39(6):200-203.(in Chinese)
[16] KORPYSA-DZIRBA W,OSEK J.Molecular characterization of enterotoxigenic Staphylococcus aureus isolated from raw cow milk in Poland[J]. Foodborne Pathogens and Disease, 2019,16(2):114-118.
[17] LIU H,LI S,MENG L,et al.Prevalence,antimicrobial susceptibility,and molecular characterization of Staphylococcus aureus isolated from dairy herds in Northern China[J]. Journal of Dairy Science, 2017,100(11):8796-8803.
[18] 孙冰清,姜芹,张妤,等.上海地区生鲜乳中金黄色葡萄球菌分离及耐药性研究[J].中国乳品工业,2020,48(2):24-26. SUN B Q,JIANG Q,ZHANG Y,et al.Study on isolation and drug resistance of Staphylococcus aureus from raw milk in Shanghai[J]. China Dairy Industry,2020,48(2):24-26.(in Chinese)
[19] 柳海宾,张燕,杜欣军等.生鲜乳中金黄色葡萄球菌检测及性状分析[J].中国乳品工业,2017,45(9):20-21. LIU H B,ZHANG Y,DU X J,et al.Prevalence and characterization of Staphylococcus aureus isolated from raw milk[J]. China Dairy Industry,2017,45(9):20-21.(in Chinese)
[20] 冯疆蓉.甘肃省生鲜乳微生物质量安全评价及有害细菌来源分析[D].兰州:兰州大学,2017. FENG J R.Microbial quality and safety evaluation of fresh milk in Gansu province and source analysis of harmful bacteria[D].Lanzhou:Lanzhou University,2017.(in Chinese)
[21] SHI C,YU Z,HO H,et al.Occurrence,antimicrobial resistance patterns,and genetic characterization of Staphylococcus aureus isolated from raw milk in the dairy farms over two seasons in China[J]. Microbial Drug Resistance,2021,27(1):99-110.
[22] 宋淑英.抚顺某奶牛场隐性乳房炎病原菌耐药性的调查研究[J].现代畜牧兽医,2022,3:56-59. SONG S Y.Investigation and research on antibiotics resistance of bacteria obtained from subclinical mastitis in a dairy farm in Fushun[J]. Modern Journal of Animal Husbandry and Veterinary Medicine,2022,3:56-59.(in Chinese)
[23] 张楠.致奶牛乳房炎金黄色葡萄球菌敏感中药有效成分筛选及应用研究[D].邯郸:河北工程大学,2021. ZHANG N.Study on screening and application of effective components of traditional Chinese medicine causing cow mastitis and Staphylococcus aureus susceptibility[D].Handan:Hebei Engineering University,2021.(in Chinese)
[24] MPHAHLELE M P,OGUTTU J W,PETZER I M,et al.Prevalence and antimicrobial drug resistance of Staphylococcus aureus isolated from cow milk samples[J]. Veterinary World, 2020,13(12):2736-2742.
[25] 孙雅君.奶牛乳腺炎源金黄色葡萄球菌耐药性相关基因的检测与耐药菌株表型异质性分析[D].杨凌:西北农林科技大学,2021. SUN Y J.Detection of drug resistance related genes and phenotypic heterogeneity of Staphylococcus aureus from cow mastitis[D].Yangling:Northwest A & F University,2021.(in Chinese)
[26] 郭英,滕达,王秀敏,等.抗菌肽NZ2114对奶牛乳房炎源金黄色葡萄球菌细胞膜、基因组和耐药性的影响[J].中国畜牧兽医,2019,46(4):1181-1190. GUO Y,TENG D,WANG X M,et al.Effect of antimicrobial peptide N22114 on cell membrane,genome and antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis milk[J]. China Animal Husbandry & Veterinary Medicine,2019,46(4):1181-1190.(in Chinese)
[27] 乔壮.不同来源肠道金黄色葡萄球菌的流行病学研究[D].扬州:扬州大学,2021. QIAO Z.Epidemiological study on intestinal Staphylococcus aureus from different sources[D].Yangzhou:Yangzhou University,2021.(in Chinese)

Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus in Raw Milk in Some Areas of Xinjiang

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