猪链球菌临床分离株对四环素类抗生素的耐药性和耐药基因分析

doi:10.16431/j.cnki.1671-7236.2022.07.038

Abstract

Abstract: 【Objective】 This study was aimed to learn the resistance of clinical isolates of Streptococcus suis in Guangdong to tetracyclines and the carriage of resistance gene.【Method】 The drug-sensitive disk diffusion method was used to analyze the tetracycline antibiotics resistance of 34 strains of Streptococcus suis isolated from Guangdong during 2016 to 2020.The tetracycline resistance genes tetA,tetC,tetD,tetM,tetO and tetX were detected by PCR.【Result】 The resistance rate of 34 strains of Streptococcus suis to tetracycline was as high as 100% (34/34),followed by minocycline 82.4% (28/34) and doxycycline 47.1% (16/34).Among 34 strains of Streptococcus suis,15 strains were triple-resistant,accounting for 44.1% (15/34),14 strains were double-resistant,accounting for 41.1% (14/34),and 5 strains were resistant to one drug,accounting for 14.7% (5/34).The results of drug resistance gene detection showed that the carrying rates of tetA,tetC,tetD,tetM,tetO and tetX genes were 0,0,0,14.7% (5/34),100% (34/34) and 0,respectively.【Conclusion】 The resistance rate of tetracycline antibiotics in clinical isolates of Streptococcus suis in Guangdong was relatively high,and the main tetracycline resistance genes carried were tetO and tetM genes.

Key words: Streptococcus suis; tetracycline antibiotics; resistance; resistance gene

CLC Number:

S852.61^＋1

LIU Baoling, CHU Pinpin, LI Chunling, YANG Dongxia, JIANG Zhiyong, ZHANG Kunli, SONG Shuai, GOU Hongchao, CAI Rujian. Analysis of Resistance and Resistance Genes of Streptococcus suis Clinical Isolates to Tetracyclines[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2796-2804.

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Analysis of Resistance and Resistance Genes of Streptococcus suis Clinical Isolates to Tetracyclines

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