炭疽芽孢杆菌携带前噬菌体的预测及耐药性与毒力分析

doi:10.16431/j.cnki.1671-7236.2023.06.041

Abstract

Abstract: 【Objective】 The purpose of the experiment was to predict and analyze the proportion of Bacillus anthracis (B.anthracis) carrying prophage and the situation of prophage carrying antibiotic resistance gene and virulence gene, to understand and analyze the influence of B.anthracis prophage on bacterial resistance and virulence, and to provide reference for the subsequent research and application of B.anthracis prophage.【Method】 The online analysis software PHASTER was used to predict the prophages carried by B.anthracis, the comprehensive antibiotic research database (CARD) and virulence factors database (VFDB) were used to predict the antibiotic resistance genes and virulence factors carried by the prophages.【Result】 1 421 B.anthracis prophages were predicted, of which 267 were intact prophages, 321 were questionable prophages and 833 were incomplete prophages.The percentage of the prophage genome ranged between 3% to 4% in each B.anthracis genome.The 771 prophages carried 1 075 drug resistance genes.There were 29 resistance phenotypes, from 28 different resistance families, covering 6 antibiotic resistance mechanisms of action.The 394 prophages encoded 688 virulence factors, grouped into 71 virulence genes and 52 virulence factors.Analysis of the composition of possible host strain sources for the virulence factor revealed that, in addition to B.anthracis, Legionella pneumophila subsp., Bacillus cereus and Bacillus thuringiensis also had a high structural proportion as possible host sources for the virulence factor.【Conclusion】 B.anthracis commonly carried prophages, but the prophage genome made up a low proportion of the B.anthracis genome.Approximately 54% prophages carried antibiotic resistance genes, dominated by phosphonic acid and β-lactam.About 28% prophages carried virulence genes.Prophages might play a role in the acquisition and transmission of antibiotic resistance genes, virulence genes and the evolution of pathogenicity in B.anthracis.

Key words: Bacillus anthracis; prophage; antibiotic resistance gene; virulence factor

CLC Number:

S852.61

CHEN Jianshi, ZHANG Ning, PU Fangxiong, MIAO Jianjia, ZENG Nan, LI Bingxue. Prediction of Bacillus anthracis Carrying Prophages and Analysis of Drug Resistance and Virulence[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2573-2584.

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Prediction of Bacillus anthracis Carrying Prophages and Analysis of Drug Resistance and Virulence

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