一株草鱼维氏气单胞菌的分离鉴定及毒力基因分析

doi:10.16431/j.cnki.1671-7236.2020.03.024

Abstract

Abstract: In order to identify the pathogenic factors for the occurrence and death of grass carp of a farm in Qingong county,Guizhou province,this study identified the pathogens through clinical anatomical observation,bacterial isolation and culture,gram staining microscopic examination,biochemical identification,drug sensitivity test,animal regression test,16S rDNA and gyr B gene sequencing and systematic analysis.The results showed that a pathogenic bacteria strain GZQG2019 was isolated from the pathogenic grass carp.The isolated bacteria presented a round,moist and smooth surface with neat and translucent edges in the culture medium,and showed a beta hemolysis ring around the colony.Gram's microscopic examination showed that the isolated bacteria were obtuse short negative bacilli at both ends.The 16S rDNA and gyr B gene sequence analysis showed that the isolates and Aeromonas veronii converged into one branch,and the homology was more than 99%.The results of drug sensitivity test showed that among the 20 antibiotics,the bacteria was sensitive to 10 drugs,such as tetracycline,butylamicarine,polycolistin B and ceftriaxone,resistant to 2 drugs,such as cefalexin and compound xinomin,and moderately sensitive to 8 drugs,such as benzacillin,penicillin,amoxicillin and gentamicin.The results of animal regression showed that the bacteria was highly pathogenic to healthy grass carp.The amplification results of 10 virulence genes showed that the isolated bacteria carried 7 virulence genes including aerolysin,heat-unstable enterotoxin,hemolysin,ribozyme,serine protease,elastase and esterase.In this study,a strain of Aeromonas veronii from grass carp was successfully isolated with strong virulence and multiple virulence factors.The drug sensitivity test results provided a reference for clinical drug use of Aeromonas veronii with sensitivity to tetracycline and butanecanil and resistance to tetracycline and butanecanil.

Key words: grass carp; Aeromonas veronii; virulence factor

CLC Number:

R378.3

ZHANG Piao, HU Andong, YANG Xia, PAN Jimai, JIANG Haibo, WEN Ming. Isolation Identification and Virulence Gene Analysis of a Grass Carp Aeromonas veronii[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 848-855.

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Isolation Identification and Virulence Gene Analysis of a Grass Carp Aeromonas veronii

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