3株维氏气单胞菌耐药性及毒力基因的比较

doi:10.16431/j.cnki.1671-7236.2020.09.033

Abstract

Abstract: To find out whether there were differences in drug resistance and virulence genes of Aeromonas veronii (A.veronii) from different regions,this study compared morphology,biochemistry test,drug sensitive test,artificial infection test,16S rDNA gene sequencing and virulence genes homology analysis for grass carp source strain GZCY2019,hybrid sturgeon source strain GZXY2018 and Ictalurus punctatus source strain GZBD2017.The results showed that all the three strains of A.veronii from different sources presented round,moist and smooth gray and translucent colonies with neat edges on the blood plate,and the colonies were surrounded by β hemolytic rings.Gram staining showed that negative Bacillus crassus at both ends.Drug sensitivity test showed that the three strains were all sensitive to butylaminacana,doxycycline,but were all intermediary to compound xinnomin,ceftadime,polymyxin B and other drugs,while were at different degrees of sensitivity or resistance to other drugs.Artificial infection test showed that the cumulative death rates of GZCY2019 and GZBD2017 were both 100%,while the rate of GZXY2018 was 80%.The homology analysis of 16S rDNA showed that the homology between GZCY2019 and GZBD2017 was 99.9%,while the homology between the former two strains and GZXY2018 was 97.1%.Virulence gene test results showed that Alt,hlyA and Fla virulence genes were all carried,while cytotoxic enterotoxin Act and Ast virulence genes were different.In this study,through comparison of multiple experiments with different sources of A.veronii,it was found that the three strains were basically similar in growth morphology,staining microscopy and biochemical results,but there were significant differences in resistance and virulence genes.

Key words: grass carp; hybrid sturgeon; Ictalurus punctatus; Aeromonas veronii

CLC Number:

S855.3

ZHANG Piao, HU Andong, YANG Xia, PAN Jimai, LIU Yanhan, ZENG Maoqin, CHENG Zhentao, JIANG Haibo, WEN Ming. Comparison of Drug Resistance and Virulence Genes of Three Strains of Aeromonas veronii[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2979-2987.

References

[1] DONOHUE M J,BEST J M,SMALLWOOD A W,et al.Differentiation of Aeromonas isolated from drinking water distribution systems using matrix-assisted laser desorption/ionization-mass spectrometry[J].Analytical Chemistry,2007,79(5):1939-1946.
[2] FANG H M,LING K C,GE R,et al.Enhancement of protective immunity in Blue gourami,Trichogaster tric-hopterus (Pallas),against Aeromonas hydrophila and Vibrio anguillarum by Aeromonas hydrophila major adhesin[J].Journal of Fish Diseases,2008,23(2):137-145.
[3] 葛铮.不同淡水鱼类维氏气单胞菌的分离鉴定及耐药性分析[D].长春:吉林农业大学,2015. GE Z.Isolated identification and drug resistance analysis of Aeromonas veronii from different freshwater fish species[D].Changchun:Jilin Agricultural Univer-sity,2015.(in Chinese)
[4] GALINDO C L,GUTIERREZ C J,CHOPRA A K.Potential involvement of galectin-3 and SNAP23 in Aeromonas hydrophila cytotoxic enterotoxin-induced host cell apoptosis[J].Microbial Pathogenesis,2006,40(2):56-68.
[5] MALTZ M,GRAF J.The type Ⅱ secretion system is essential for erythrocyte lysis and gut colonization by the leech digestive tract symbiont Aeromonas veronii[J].Applied and Environmental Microbiology,2011,77(2):597-603.
[6] SEN K,LYE D.Importance of flagella and enterotoxins for Aeromonas virulence in a mouse model[J].Canadian Journal of Microbiology,2007,53(2):261-269.
[7] 康元环,张冬星,杨滨僮,等.维氏气单胞菌最新研究进展[J].中国人兽共患病学报,2018,34(5):452-459. KANG Y H,ZHANG D X,YANG B T,et al.Latest research progress on Aeromonas veronii[J].Chinese Journal of Zoonoses,2018,34(5):452-459.(in Chinese)
[8] 贾俊鹏,康元环,张冬星,等.锦鲫致病性维氏气单胞菌的分离鉴定及耐药性分析[J].中国预防兽医学报,2018,40(1):19-23. JIA J P,KANG Y H,ZHANG D X,et al.Isolation,identification and drug susceptibility of the pathogenic Aeromonas veronii from Brocaded crucian[J].Chinese Journal of Preventive Veterinary Medicine,2018,40(1):19-23.(in Chinese)
[9] YAMAMOTO S,HARAYAMA S.PCR amplification and direct sequencing of gyrB gene with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains[J].Applied and Environmental Microbiology,1995,61(10):3768.
[10] NAWAZ M,KHAN S A,KHAN A A,et al.Detection and characterization of virulence genes and integrons in Aeromonas veronii isolated from catfish[J].Food Microbiology,2010,27(3):327-331.
[11] 王闻卿,王多春,朱林英,等.上海市浦东新区腹泻患者气单胞菌流行特征及毒力基因研究[J].中华流行病学杂志,2016,37(3):402-405. WANG W Q,WANG D C,ZHU L Y,et al.Infection status and virulent genes of Aeromonas in diarrhea patients in Pudong New Area,Shanghai[J].Chinese Journal of Epidemiology,2016,37(3):402-405.(in Chinese)
[12] DALLAL M M S,FARD R M N,TALKHABI M K,et al.Prevalence,virulence and antimicrobial resistance patterns of Aeromonas spp.isolated from children with diarrhea[J].Germs,2016,6(3):91-96.
[13] 张海月,康元环,吴同垒,等.狐源维氏气单胞菌体内诱导基因的筛选[J].中国兽医科学,2017,47(1):54-59. ZHANG H Y,KANG Y H,WU T L,et al.Screening of in vivo-induced genes of Aeromonas veronii isolated from fox[J].Chinese Veterinary Science,2017,47(1):54-59.(in Chinese)
[14] 高彩霞,任燕,王庆,等.草鱼源致病性维氏气单胞菌的分离鉴定及药物敏感性分析[J].安徽农业大学学报,2018,45(3):409-415. GAO C X,REN Y,WANG Q,et al.Isolation identification and antimicrobial susceptibility of pathogenic Aeromonas veronii isolated from grass carp[J].Journal of Anhui Agricultural University,2018,45(3):409-415.(in Chinese)
[15] 王兴丽,汪开毓,陈德芳,等.一株虹鳟源维氏气单胞菌的分离鉴定及组织病理学观察[J].中国预防兽医学报,2016,38(11):879-883. WANG X L,WANG K Y,CHEN D F,et al.Isolation and identification of Aeromonas veronii from rainbow trout and histopathology study of the diseased fish[J].Chinese Journal of Preventive Veterinary Medicine,2016,38(11):879-883.(in Chinese)
[16] 方振华,曾玉梅,洪美玲,等.中华条颈龟致病性维氏气单胞菌的分离鉴定及药敏试验[J].中国畜牧兽医,2019,46(9):2760-2768. FANG Z H,ZENG Y M,HONG M L,et al.Identification and susceptibility test of pathogenic Aeromonas veronii isolated from mauremys sinensis[J].China Animal Husbandry & Veterinary Medicine,2019,46(9):2760-2768.(in Chinese)
[17] 周慧华,黄晓东,安健,等.中华绒螯蟹致病性维氏气单胞菌的分离鉴定、药敏特性及其组织病理学观察[J].南方农业学报,2019,50(8):1851-1859. ZHOU H H,HUANG X D,AN J,et al.Isolation,identification and antibiotic susceptibility of pathogenic Aeromonas veronii in Eriocheir sinensis and its histopathological observations[J].Journal of Southern Agriculture,2019,50(8):1851-1859.(in Chinese)
[18] 田照辉,徐绍刚,胡红霞,等.西伯利亚鲟MHCⅡβ基因克隆、组织分布及细菌感染后的表达变化[J].大连海洋大学学报,2019,34(1):56-62. TIAN Z H,XU S G,HU H X,et al.Molecular cloning and expression of major histocompatibility complex class Ⅱβ gene in tissues of Siberian sturgeon Acipenser baerii[J].Journal of Dalian Ocean University,2019,34(1):56-62.(in Chinese)
[19] 彭博文,杨移斌,艾晓辉,等.克氏原螯虾源维氏气单胞菌分离鉴定及药敏特性研究[J].海洋湖沼通报,2018,4:108-114. PENG B W,YANG Y B,AI X H,et al.Isolation and identification of Aeromonas veronii from Procambarus clarkii and its antibiotic sensitivity[J].Transactions of Oceanology and Limnology,2018,4:108-114.(in Chinese)
[20] 任燕,高彩霞,曾伟伟.两株草鱼源维氏气单胞菌菌株的主要表型特征及致病力比较[J].中国预防兽医学报,2019,41(2):145-150. REN Y,GAO C X,ZENG W W,et al.Pathogenicity and main phenotype characteristics of two Aeromonas veronii isolated from diseased grass carp[J].Chinese Journal of Preventive Veterinary Medicine,2019,41(2):145-150.(in Chinese)
[21] 崔佳佳,李绍戊,王荻,等.三北地区鱼源气单胞菌的分离鉴定与药敏试验[J].江西农业大学学报,2016,38(1):152-159. CUI J J,LI S W,WANG D,et al.Isolation,identification and susceptibility test of Aeromonas from fish in the three north areas of China[J].Acta Agriculturae Universitatis Jiangxiensis,2016,38(1):152-159.(in Chinese)
[22] 吴雅丽,邓玉婷,姜兰,等.广东省水产动物源气单胞菌对抗菌药物的耐药分析[J].上海海洋大学学报,2013,22(2):219-224. WU Y L,DENG Y T,JIANG L,et al.Antimicrobial susceptibilities of Aeromonas strains isolated from various aquatic animals in Guangdong province[J].Journal of Shanghai Ocean University 2013,22(2):219-224.(in Chinese)
[23] 赵敏.四川地区斑点叉尾鮰源维氏气单胞菌的耐药性以及耐药基因检测分析[D].雅安:四川农业大学,2013. ZHAO M.Antimicrobial agents resistance and its genetic determinants in Aeromonas veronii isolated from Channel Catfish (Ictalurus punctatus)[D].Ya'an:Sichuan Agricultural University,2013.(in Chinese)
[24] 李伟杰,赵耘,刘燕,等.狐狸源致病性维氏气单胞菌的分离鉴定及耐药性分析[J].中国预防兽医学报,2012,34(4):289-292. LI W J,ZHAO Y,LIU Y,et al.Isolation and identification of pathogenic Aeromonas veronii from fox[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(4):289-292.(in Chinese)

Comparison of Drug Resistance and Virulence Genes of Three Strains of Aeromonas veronii

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