类志贺邻单胞菌感染杂交鲟肠道组织转录组分析

doi:10.16431/j.cnki.1671-7236.2020.09.005

Abstract

Abstract: In order to explore the changes of intestinal transcriptome of sturgeons infected with pathogenic bacteria,the hybrid sturgeons about 360 d were infected by Plesiomonas shigelloides,and their intestinal tissues were collected after 24 h of inoculation and used for extraction of mRNA,and transcriptome sequencing was performed using high-throughput sequencing technology and analyzed for GO function classification and KEGG signal pathway.The results showed that there were 13 542 of differentially expressed genes with 9 774 of up-regulated genes and 3 768 of down-regulated genes in the hybrid sturgeon intestinal comparing with the control group.GO analysis found that significantly enriched sturgeons gut differentially expressed genes were mainly involved in immune system process,immune effect process and stimulation response.Significant enrichment of molecular functions mainly included:DNA binding,signal receptor activity,nucleic acid transcription factor activity.The cell components were mainly extracellular,extracellular components,cell membrane.KEGG analysis showed that the immune signaling pathways involved in differentially expressed genes in sturgeons intestinal were RIG-Ⅰ-like receptor signaling pathway,Toll-like receptor signaling pathway and cytosolic DNA-sensing pathway.These results laid a good foundation for the defense molecular mechanism of the hybrid sturgeon against pathogenic infection.

Key words: hybrid sturgeon; intestinal tissue; high-throughput sequencing; transcriptome; Plesiomonas shigelloides

CLC Number:

S852.61⁺2

ZHANG Mingyang, ZENG Maoqin, LIU Yanhan, ZHANG Piao, YANG Xia, YANG Ying, WEN Guilan, CHENG Zhentao, JIANG Haibo, WEN Ming. Transcriptome Analysis of Hybrid Sturgeons Intestinal Infected with Plesiomonas shigelloides[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2732-2740.

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Transcriptome Analysis of Hybrid Sturgeons Intestinal Infected with Plesiomonas shigelloides

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