不同氧浓度下猪睾丸间质细胞转录组分析

doi:10.16431/j.cnki.1671-7236.2023.06.002

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the difference in the expression profile of porcine Leydig cells under different oxygen concentrations and screen the key genes of hypoxia leading to the damage of porcine Leydig cells.【Method】 Porcine Leydig cells were divided into hypoxia group (1% oxygen, H24 group) and normal group (15.75% oxygen, N24 group) with 3 replicates in each group.The cells were treated for 24 h under corresponding conditions, and the cell survival rate was detected by CCK8 method.Transcriptome sequencing technology was used to screen out the differentially expressed genes in Leydig cells of hypoxia and normal oxygen groups.DESeq 2.0 software was used to analyze the differentially expressed genes, and GO function and KEGG pathway enrichment analysis were performed on the differentially expressed genes, so as to further screen the regulatory genes related to oxygen content.【Result】 After 24 h hypoxia stimulation, the survival rate of porcine Leydig cells in hypoxia group was extremely significantly decreased compared with normal group (P<0.01).A total of 1 654 differentially expressed genes were obtained by transcriptome sequencing, among which 1 242 genes were up-regulated and 412 genes were down-regulated.GO function enrichment analysis showed that 8 enrichment items were obtained, including 1 biological process and 7 molecular functions.KEGG pathway enrichment analysis showed that 10 signaling pathways were significantly enriched, including HIF-1 signaling pathway, JAK-STAT signaling pathway and AGE-RAGE signaling pathway in diabetic complications, etc.Six genes related to hypoxia stress were obtained by enrichment analysis of GO function and KEGG pathway, including signal transducer and activator of transcription 5(STAT5), interleukin-8 (IL-8), C-C motif chemokine ligand 2 (CCL2), interferon alpha and beta receptor subunit 1 (IFNAR1), suppressor of cytokine signaling 1 (SOCS1) and erythropoietin (EPO).【Conclusion】 In this study, six genes related to hypoxia injury and stress repair in porcine Leydig cells (SOCS1, IFNAR1, EPO, STAT5, IL-8 and CCL2 genes) were obtained through transcriptomic analysis of porcine Leydig cells under different oxygen concentrations, providing reference for the study on the low reproductive ability of boar in plateau area.

Key words: hypoxia; porcine Leydig cells; reproductive injury; transcriptome sequencing

CLC Number:

S828

CHEN Kaiwen, ZHAO Yunjiao, WANG Lei, ZHANG Jianbo, MA Yuhong, WU Guofang. Transcriptome Analysis of Porcine Leydig Cells Under Different Oxygen Concentrations[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2185-2195.

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Transcriptome Analysis of Porcine Leydig Cells Under Different Oxygen Concentrations

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