牦牛低氧诱导因子-1α基因克隆及蛋白质结构分析

doi:10.16431/j.cnki.1671-7236.2016.06.005

Abstract

Abstract: In this study,hypoxia inducible factor-1α(HIF-1α) gene was cloned from yak spleen by RT-PCR,and it was divided into two sections for amplification.The sequence and protein structure were analyzed using related bioinformatics tools.The length of the coding region was 2 472 bp,which encoded 823 amino acids.Its molecule mass was 92.13 ku,and PI was 5.09.It displayed as a hydrophilic protein,whose secondary structure was mainly composed of random coil and α-helix.It had 69 phosphorylation sites and 4 N-glycosylation sites.Phylogenetic tree displayed that Maiwa yak,Bos grunniens,Bos mutus and Bos taurus gathered into one cluster.Maiwa yak HIF-1α gene was successfully cloned in this study,it provided a viable reference for further study of genetic characteristics of HIF-1α.

Key words: Maiwa yak; hypoxia inducible factor-1α; cloning; protein analysis

CLC Number:

Q785

DUAN Hui-qin, WANG Li, LI Jian, XIONG Xian-rong. Cloning and Protein Structure Analysis of Hypoxia Inducible Factor-1α Gene in Yak[J]. , 2016, 43(6): 1430-1436.

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Cloning and Protein Structure Analysis of Hypoxia Inducible Factor-1α Gene in Yak

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