A型塞内卡病毒感染PK-15细胞环状RNA表达谱分析

doi:10.16431/j.cnki.1671-7236.2023.01.028

Abstract

Abstract: 【Objective】 The purpose of the test was to analyze the effect of Seneca virus A (SVA) infection on the expression profile of circular RNAs (circRNAs) in pig renal epithelial cells (PK-15), and to explore the potential regulatory role of circRNAs during SVA infection.【Method】 The circRNAs transcriptome of SVA-infected and control PK-15 cells were sequenced based on the Illumina HiSeq 2000 platform.The source genes of the differentially expressed circRNAs were subjected to GO function and KEGG pathway enrichment analysis, and the expression levels of the newly discovered circRNAs were identified by Real-time quantitative PCR.【Result】 Transcriptome sequencing results revealed that a total of 432 novel circRNAs were identified in SVA-infected and control PK-15 cells.In SVA-infected PK-15 cells, the expression levels of 87 circRNAs were significantly up-regulated and 74 circRNAs were significantly down-regulated compared to control PK-15 cells. GO functional analysis of the source genes of differentially expressed circRNAs indicated that the differentially expressed circRNAs source genes in SVA-infected PK-15 cells were mainly enriched in the nucleolus, organelle membranes, and physiological processes such as cellular macromolecular metabolic processes and development.The results of KEGG pathway enrichment analysis showed that the significantly differentially expressed circRNAs source genes in the SVA-infected PK-15 cells were mainly enriched in the cytokinesis process, cAMP signaling pathway, Rap1 signaling pathway and Ras signaling pathway.The results of Real-time quantitative PCR verification of the 6 significantly differentially expressed circRNAs demonstrated that their expression levels were consistent with the high-throughput sequencing.【Conclusion】 This was the first report of differential analysis of the circRNAs expression profiles of SVA-infected PK-15 cells.It was found that the differentially expressed circRNAs were widely involved in macromolecular metabolism, cytokinesis and cell proliferation, adhesion and antiviral processes, and this study provided a reference for the further exploration of the molecular mechanism of circRNAs during SVA-infection.

Key words: circular RNA (circRNA); Seneca virus A (SVA); high-throughput sequencing; PK-15 cell

CLC Number:

S852.65⁺9.6

CHEN Yanxi, WANG Chen, LUO Yuan, ZHOU Yuancheng, XU Zhiwen, PENG Yuanyi, SONG Zhenhui, LIU Xiao. circRNA Expression Profile Analysis of PK-15 Cells Response to Seneca Virus A Infection[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 280-289.

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circRNA Expression Profile Analysis of PK-15 Cells Response to Seneca Virus A Infection

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