China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (2): 734-744.doi: 10.16431/j.cnki.1671-7236.2023.02.031

• Basic Veterinary Medicine • Previous Articles     Next Articles

Screening of the Natural Products Against Senecavirus A and Study on Its Mechanism of Antagonism

MENG Yingwen1,2, LI Yuehua2, SHA Zhou2,3, LI Chenyu2,4, GONG Youquan2,5, DONG Yaqin2,3, WEI Rong2,3, ZOU Fengcai1, NI Bo2,3   

  1. 1. College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China;
    2. China Animal Health and Epidemiology Center, Qingdao 266032, China;
    3. Key Laboratory of Animal Biosafety Risk Prevention and Control (South), Ministry of Agriculture and Rural Affairs, Qingdao 266032, China;
    4. College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030800, China;
    5. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163711, China
  • Received:2022-09-13 Online:2023-02-05 Published:2023-02-06

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Abstract: 【Objective】 The purpose of this study was to screen the natural products with anti-SVA activity from natural products library and to discover the mechanism of their antagonism.【Method】 Incorporating the use of the luciferase report technology,the high throughput platform for in vitro screening of anti-SVA natural products was established.In this platform,recombinant Luciferase reporter virus (rSVA-NLuc) system and BHK-21 cell were used to rapidly screen natural products against SVA from natural products library.The natural products with inhibitory effect on luciferase activity at the concentration of 10 μmol/L were screened from the natural product library,and their inhibitory activity was further verified by quantitative Real-time RT-PCR,at the same time,maximal nontoxic concentrations were determined by cytotoxicity test.The anti-viral mechanisms,which covering 4 main processes of the virus infection cycle including adsorption,entry,replication,assembly and release,were further studied with quantitative Real-time RT-PCR and 50% tissue culture infective dose(TCID50).【Result】 16 natural products against SVA were screened from a library containing 560 natural products.Four safe and effective molecules were identified by quantitative Real-time RT-PCR and cytotoxicity test,namely (20S)-protopanaxatriol,paxilline,fangchinoline and hypocrellin B.Among them,(20S)-protopanaxatriol could inhibit the adsorption,replication of SVA.Paxilline could inhibit the adsorption,entry,replication,assembly and release of SVA.Fangchinoline mainly inhibited the entry and replication of SVA.Hypocrellin B could inhibit the adsorption,entry,replication,assembly and release of SVA.【Conclusion】 A total of 4 natural products against SVA were screened out from the natural products library.These compounds had good antiviral activity and different anti-viral mechanism.This study provided an important reference for the further development of anti SVA drugs.

Key words: Seneca virus A (SVA); natural product; high-throughput screening; antagonism mechanism

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