犬猫皮肤病细菌性病原分离鉴定及天然产物抑菌效果分析

doi:10.16431/j.cnki.1671-7236.2022.04.038

Abstract

Abstract: 【Objective】 The purpose of the experiment was to isolate and identify the bacterial pathogens of dogs and cats suffering from skin diseases in Wuhan,and to explore their sensitivity to traditional antibiotics and natural active products gambogic acid (GA) and 6-bromoindirubin-3’-oxime (BIO).【Method】 Skin pathogen bacteria from dogs and cats were collected and isolated,as well as growth characteristics,Gram staining microscope observation and PCR were determined.SPF mice were used to verify pathogenicity.The drug resistance to traditional drugs was verified by drug sensitive paper,and the minimum inhibitory concentration (MIC) of natural products was measured.【Result】 2 strains of Staphylococcus aureus,3 strains of Staphylococcus pseudointermediate,2 strains of Staphylococcus felis,1 strain of Streptococcus canis,and 1 strain of Proteus mirabillis were isolated.The pathogenicity was verified by skin infection in SPF mice.Streptococcus canis and Proteus mirabillis were sensitive to the tested drugs.Staphylococcus aureus had different resistance to cotrimoxazole,penicillin,erythromycin,tetracycline,levofloxacin,oxacillin,gentamicin,clindamycin and chloramphenicol.The natural active products of GA and BIO had good antibacterial effects on the pathogenic bacteria,and except strain F5,the MIC of GA was universally lower than that of BIO.【Conclusion】 In the study,a total of 9 pathogenic bacteria were isolated from dogs and cats.Streptococcus canis and Proteus mirabilis were sensitive to traditional antimicrobial agents,and some Staphylococcus strains were resistant to them.GA and BIO had obvious antibacterial effects against skin pathogenic bacteria.It showed that GA and BIO could be used as potential antibacterial drugs to prevent and control skin bacterial disease in dogs and cats.

Key words: dogs and cats; skin disease; pathogenic bacteria; isolation and identification; natural products; antibacterial effect

CLC Number:

S852.61

ZHAO Tianrui, SHAO Jinhua, WANG Chaohao, YAN Rui, LEI Yifei, LIU Xiaoya, HU Changmin. Isolation and Identification of Skin Pathogenic Bacteria from Dogs and Cats and Analysis of Antibacterial Effect of Natural Products[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1556-1567.

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Isolation and Identification of Skin Pathogenic Bacteria from Dogs and Cats and Analysis of Antibacterial Effect of Natural Products

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