China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (1): 398-407.doi: 10.16431/j.cnki.1671-7236.2023.01.040

• Basic Veterinary Medicine • Previous Articles     Next Articles

Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer

YANG Fumei1, YANG Linfu1, ZENG Cheng2, YANG Zhi1, WANG Kang1, DUAN Gang1, DAI Feiyan1   

  1. 1. College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China;
    2. Kunming Endangered Animal and Plant Collection and Rescue Center, Kunming 650201, China
  • Received:2022-08-29 Online:2023-01-05 Published:2023-01-04

Abstract: 【Objective】 The purpose of the experiment was to explore the biological characteristics of potential microorganisms in the dead barking deer in the Jiaozi Snow Mountain Nature Reserve, Kunming, reveal whether there was a correlation between them and the death of the barking deer, and to provide data support for microbial diversity in Kunming Jiaozi Snow Mountain Nature Reserve.【Method】 Part of the muscle tissue of the dead barking deer was collected aseptically in the experiment.The bacteria were isolated and cultured.After identification by Gram staining microscopy, biochemical test and 16S rRNA gene amplification, sequence comparison was conducted and phylogenetic tree was constructed to determine the type of the isolated strain, and drug sensitivity test and animal regression test were conducted on the isolated strain.【Result】 The colony morphology of the isolated strain on TSA and blood agar medium was round, with neat edges, smooth surface and milky white color, it did not grow on MAC medium, Gram staining microscope showed purple bacilli.The biochemical test results showed that, the isolated strain could not ferment sucrose, raffinose, sorbitol, etc., phenylalanine, ornithine, lysine, V-P test, and glucose gas production were positive.The 16S rRNA gene sequence and phylogenetic tree analysis showed that the strain was 100% similar to the Carnobacterium maltaromaticum (C.maltobotulinum) strain MMF-23 (GQ304931.1) in the GenBank.Combined with morphological, biochemical features, the isolate was identified as C.maltaromaticum, named BJT86, and the 16S rRNA sequence was submitted to GenBank to obtain the accession number ON966116.1.The results of drug sensitivity test showed that the isolated strain was resistant to 6 kinds of antibiotics such as penicillin, amoxicillin and piperacillin, and sensitive to 18 kinds of antibiotics such as cefazolin, cefotaxime and cefsulbactam.Animal regression test showed that the isolated strain was not pathogenic.【Conclusion】 In this experiment, a strain of C.maltaromaticum was successfully isolated for the first time from the muscle tissue of the dead barking deer in the Jiaozi Snow Mountain Nature Reserve in Kunming, which could provide a new reference direction for food safety and basic data for the preparation of probiotics.

Key words: Carnobacterium maltaromaticum; isolation and identification; drug susceptibility test; regression test

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