基于高通量测序技术的敖鲁古雅驯鹿鹿茸转录组分析

doi:10.16431/j.cnki.1671-7236.2020.07.002

Abstract

Abstract: This experiment was aimed to obtain the genetic information of reindeer and the transcriptome characteristics of antler tissue by high-throughput sequencing technology,and further explore the key gene information related to the economic traits of antler.RNA was extracted from antler tissue in reindeer,and then the purity,integrity and contamination of RNA was detected.Qualified RNA was used for library preparation.The library preparation needs to be tested,after the test,transcriptome sequencing analysis of antler in reindeer using the Illuminanovaseq platform.Functional annotation,gene abundance analysis and other analysis of the transcriptome sequences of antler in reindeer were performed using software such as Diamond,HMMER,KAAS and Blast2GO.The results showed that 47 818 202 raw reads were obtained by sequencing,46 964 478 clean reads were obtained after filtering and quality control,and 49 171 Unigenes were obtained after assembly,indicating that the high-quality antler transcriptome was obtained by sequencing.Based on sequence identity analysis,Unigenes were compared with NR,Swiss-Prot,KOG,KEGG,Pfam and GO databases,the annotations accounted for 89.54% successfully,with a total of 44 029 sequences.After clustering of Corset,49 171 Unigenes were obtained,and 13 795 SNPs and 18 446 SSRs were found in Unigenes.22 955 Unigenes annotated with GO terms,accouting for 46.68% of the total annotated results.Compared with the KEGG database,49 171 Unigenes might be involved in the metabolic pathway,and 20 285 Unigenes were annotated to 32 KEGG metabolic pathways.In addition,there were some high-abundance genes in transcriptome results,such as OPN, TMSB4 and TMSB10.Their function might be related to anlter in reindeer.In summary,this results not only supplement the genomic data of reindeer,but also reveal the genetic characteristics of antler in reindeer in the growing season,which provided a theoretical basis for molecular genetics research,resource conservation and utilization and restoration of population resources.

Key words: reindeer; antler; high-throughput sequencing; transcriptome

CLC Number:

JU Yan, LIU Huamiao, HE Jinming, WANG Lei, ZHAO Pei, ZHANG Ranran, DONG Yimeng, DONG Shiwu, TANG Lixin, XING Xiumei. Transcriptome Analysis of Antler in Aoluguya Reindeer Based on High-throughput Sequencing Technology[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 1981-1989.

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Transcriptome Analysis of Antler in Aoluguya Reindeer Based on High-throughput Sequencing Technology

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