不同腹脂率番鸭回肠转录组差异分析

doi:10.16431/j.cnki.1671-7236.2022.08.006

Abstract

Abstract: 【Objective】 This study was conducted to identify the major genes related to abdominal fat deposition in ileum of muscovy ducks.【Method】 200 70-day-old muscovy ducks were randomly selected from 2 000 muscovy ducks for slaughter.The abdominal fat was collected and weighed to calculate abdominal fat percentage.Sorted by abdominal fat rate,the ileum tissues of 5 muscovy ducks with the highest abdominal fat percentage and 5 muscovy ducks with the lowest abdominal fat percentage were selected for transcriptome sequencing analysis.Illumina NovaSeq 6000 high-throughput RNA-Seq sequencing technology was used to perform transcriptome sequencing on ileum of 2 kinds of muscovy ducks with abdominal fat rate.RSEM software was used to compare the sequenced reads sequence with the reference genome of Trinity to screen out differentially expressed genes.GO and KEGG databases were used for functional annotation,enrichment analysis and cluster analysis,and Pearson correlation analysis was used to analyze the correlation between abdominal fat weight and abdominal fat percentage and differentially expressed genes.【Result】 There were 602 differentially expressed genes in ileum of muscovy ducks with high and low abdominal fat rates.Compared with the muscovy ducks with low abdominal fat rate,285 genes were up-regulated and 317 genes were down-regulated in ileum of muscovy ducks with high abdominal fat rate.GO and KEGG enrichment analysis showed that there were 5 GO terms (lipid response,steroid hormone-mediated signaling,steroid hormone response,steroid hormone-stimulated cellular response,and lipid cellular response) and 4 KEGG pathways (PPAR signaling,biosynthesis of primary bile acids,arachidonic acid metabolism,and bile secretion) related to lipid metabolism.In addition,6 genes were found to be significantly correlated with fat metabolism (P<0.05),including NR5A2,ACBP,ACOX2,FABP2,FABP4 and FABP5,among which ACOX2 and FABP5 genes were significantly positively correlated with abdominal fat weight and abdominal fat percentage (P<0.05),ACBP gene was significantly positively correlated with abdominal fat weight and abdominal fat percentage (P<0.01).【Conclusion】 There were differences in the ileum transcriptome of muscovy ducks with different abdominal fat rates.It was found that 5 GO terms and 4 KEGG pathways,NR5A2,ACBP,ACOX2,FABP2,FABP4 and FABP5 genes were related to abdominal fat deposition in muscovy ducks.

Key words: muscovy ducks; abdominal fat; fat metabolism; transcriptome

CLC Number:

S815

WANG Xiaoli, WANG Xingxin, YANG Caimei, XIAO Yingping, YANG Hua, LYU Wentao. Analysis of Ileum Transcriptome Differences in Muscovy Ducks with Different Abdominal Fat Rates[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2899-2909.

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Analysis of Ileum Transcriptome Differences in Muscovy Ducks with Different Abdominal Fat Rates

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