腹泻犊牛与健康犊牛粪便菌群结构组成与功能研究

doi:10.16431/j.cnki.1671-7236.2020.09.010

Abstract

Abstract: This study was aimed to identify some specific microbiota to indicate the calf diarrhea by exploring the differences of fecal microbiota composition and function between healthy and diarrhea calves.Fecal samples were collected from 10 diarrheal and 10 healthy Xianan calves and used to extract genomic DNA.Fecal microbial diversity was investigated by 16S rRNA Illumina MiSeq high-throughput sequencing technology.Biomarkers with statistical differences were obtained,the differentially functional genes were annotated between two groups.The results showed that the fecal microbial diversity of diarrhea calves was less than healthy calves.Compared with healthy calves,the abundance of Proteobacteria and Fusobacteria was significantly increased,while Spirochaetae was decreased in calves with diarrhea at phylum level (P<0.05).At genus level,the relative abundances of Escherichia-Shigella,Fusobacterium,Lactobacillus and Clostridium_sensu_stricto_1 exhibited increased calves with diarrhea,the relative abundance of uncultured_bacterium_f_Bacteroidales_S24-7_group,Alloprevotella and Prevotellaceae_NK3B31_group exhibited decreased in calves with diarrhea (P<0.05).The differentially functional genes in diarrheal calves were significantly reduced in bacterial translation,ribosomal structure and biogenesis by COG functional profiles analyzes.The KEGG pathways was analyzed,the pathways in diarrheal calves were significantly increased in carbohydrate metabolism,xenobiotics biodegradation and metabolism,whereas they significantly decreased in cell growth,death,replication,repair,translation and transcription (P<0.05).The dominant bacteria were analyzed and ten biomarkers were identified as indicator for evaluating calf diarrhea between diarrhea and healthy groups in this study.The results provided crucial references for future research on preventing and treating calves diarrhea.

Key words: calves; fecal microbiota; diarrhea; 16S rRNA; high-throughput sequencing

CLC Number:

S814.8

ZHANG Zijing, ZHU Xiaoting, LYU Shijie, JIN Lei, XU Jiawei, HUANG Yongzhen, LI Zhiming, WANG Xianwei, YU Xiang, YANG Shang, LI Jing, WANG Eryao, XU Zhaoxue, SHI Qiaoting. Study on the Structure and Function of Fecal Microbiome Between Diarrhea and Healthy Calves[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2779-2788.

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Study on the Structure and Function of Fecal Microbiome Between Diarrhea and Healthy Calves

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