基于蛋白质组学技术分析初乳和常乳摄入对初生犊牛肝脏蛋白表达的影响

doi:10.16431/j.cnki.1671-7236.2023.01.004

Abstract

Abstract: 【Objective】 This study was aimed to analyze the proteomics of hepatic proteins in calves and screen the differential proteins related to colostrum and milk feeding.【Method】 Nine bull calves delivered by multiparity Holstein cows were randomly divided into 3 groups in this study, including 3 colostrum-fed calves slaughtered at 24 h postpartum (CI group), 3 milk-fed calves slaughtered at 24 h postpartum (M group) and 3 calves not fed any milk or colostrum slaughtered 2 h after birth (CT group).The expression of hepatic proteins in calves in 3 groups were detected using a Label-free approach, the differentially expressed proteins (DEPs) were screened with Q<0.05 as the threshold, and analyzed by GO function and KEGG pathway enrichment analysis.【Result】 The results showed that 3 901 proteins were identified, 3 521, 3 646 and 3 548 proteins were obtained from CI, M and CT groups, respectively.Of these proteins, 3 202 proteins collectively expressed in CI, M and CT groups.Moreover, 287 DEPs in liver of calves have been found to be differentially expressed between CI and M groups, and they were related to cellular metabolism, response to stress, growth and development and oxidation-reduction process, the most significantly enriched pathways were endocytosis, amino acid metabolism and oxidative phosphorylation;And 154 DEPs in liver of calves have been found to be differentially expressed between CI and M groups, which were involved in cellular metabolism, single-organism process and creatine biosynthetic process, the most enriched pathways were amino acid metabolic process, focal adhesion and endocytosis.【Conclusion】 The results revealed that the DEPs in liver of calves fed with colostrum were mainly involved in catabolism and oxidation-reduction process, which provided a new understanding of protein response mechanism in liver of calves after colostrum feeding.

Key words: colostrum; milk; calves; liver; proteomics

CLC Number:

S823

WU Tao, QI Yunxia, LIU Wei, ZHU Honglong, HUANG Dongwei, DING Haisheng, ZHAO Huiling, CHENG Guanglong, ZHAO Xiaowei. Effect of Colostrum and Milk Feeding on Hepatic Proteins of Calves Based on Proteomics Analysis[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 37-45.

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Effect of Colostrum and Milk Feeding on Hepatic Proteins of Calves Based on Proteomics Analysis

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