广西地区不同饲养模式及不同季节水牛奶霉菌毒素污染状况调查

doi:10.16431/j.cnki.1671-7236.2022.09.040

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the mycotoxin contamination of buffalo milk in different seasons under different feeding modes in Guangxi.【Method】 Two seasons from October to November 2020 (autumn) and April 2021 (spring) were selected, and eight samples of raw buffalo milk were randomly collected from three breeding modes(large-scale, breeding cooperatives or breeding communities, free-range breeding) in each season.A total of 48 samples were collected.Mycotoxin contamination of buffalo milk, such as aflatoxin M₁(AFM₁), ochratoxin A(OTA), HT-2 toxin(HT-2), T-2 toxin(T-2), α-zearalenol(α-ZEL), zearalenone(ZEN) and deoxynivalenol(DON) were determined by ELISA.【Result】 Among the 48 samples, 16 (33.33%) samples were detected AFM₁.The highest detection rate was 43.75% in farming cooperatives or farming communities, and the lowest was 18.75% in free-range farming.The content of AFM₁ in the containminated samples was lower than the national limit standard (0.5 μg/kg).Two samples (4.17%) were exceeded the European Union Standard (0.05 μg/kg).The adult provisional maximum tolerable daily intake (PMTDI) of HT-2, T-2, α-ZEL, ZEN and DON in raw buffalo milk were far lower than the set value of Joint FAO/WHO Expert Committee on Food Additives(JECFA).The adult provisional tolerable weekly intake (PTWI) of OTA in raw buffalo milk was also lower than the set value of JECFA.Moreover, the content of OTA was lower than the European limit standard (<2 ng/mL).In addition, compared with the breeding cooperatives or breeding communities mode, the content of HT-2 of raw buffalo milk produced by both free-range and large-scale modes were significantly reduced (P<0.05).The content of T-2 of raw buffalo milk produced by the large-scale mode was significantly lower than free-range mode (P<0.05).In raw buffalo milk, the average content of AFM₁ and the proportion of AFM₁ content exceeding the European limit standard in autumn were higher than those in spring, but the detection rate of AFM₁ in spring was higher than that in autumn.Among the 3 feeding modes, the detection rate of AFM₁ in the samples of free-range mode in spring and autumn was the lowest.The average contents of OTA and DON in raw buffalo milk produced by each breeding mode in autumn was higher than that in spring.【Conclusion】 The current quality of raw buffalo milk in Guangxi was safe(the content of AFM₁ was less than 0.5 μg/kg, the adult PMTDI of HT-2, T-2, α-ZEL, ZEN, DON and the adult PTWI of OTA were lower than the set value of JECFA), but a variety of mycotoxins had been detected in raw buffalo milk, and the risk of mycotoxin contamination might be a concern.

Key words: feeding mode; raw buffffalo milk; mycotoxin; ELISA method

CLC Number:

S859.84

TANG Qingfeng, LI Ling, HUANG Jiaxiang, FANG Wenyuan, NONG Haoru, TANG Yan, LU Chengwei, WEI Panqiu, FENG Ling. Survey on Mycotoxin Contamination of Buffalo Milk in Different Seasons Under Different Feeding Modes in Guangxi[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3665-3675.

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Survey on Mycotoxin Contamination of Buffalo Milk in Different Seasons Under Different Feeding Modes in Guangxi

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