中国青贮玉米中霉菌毒素的污染情况分析与动物健康风险评估

doi:10.16431/j.cnki.1671-7236.2021.12.013

Abstract

Abstract: In order to find out the pollution status of mycotoxins and its impact on animals in silage corn of China, 251 samples (90 copies of whole plant corn and 161 copies of silage corn) were collected from differernt large-scale pastures located in 28 counties of 9 provinces (autonomous regions), including Northeast (Heilongjiang), Northwest (Inner Mongolia, Shanxi), Huang-Huai-Hai (Henan, Hebei), Central (Jiangsu), Southwest (Guizhou, Sichuan) and Tibet.The contents of 12 types of mycotoxins, such as aflatoxin (AFs), vomiting toxin (DON), fumonisin B (FB) and zearalenone (ZEN) were measured and the animal health risk was assessed by exposure assessment.The results showed that:①With referencing to feed hygiene standard of China, the over-standard rate of ZEN was 1.19%.When silage corn was used as the main roughage for lactating dairy cows, the over-standard rate of FB and AFs were 23.90% and 13.94%, respectively, referencing to the feed safety standards of FDA and EC accordingly.②There was no significant difference in the content of mycotoxins between whole plant corn and whole plant corn silage (P>0.05).③The detected type and contents of mycotoxins in Huang-Huai-Hai area, Central area and Southwest area were higher, and the content was lower in Tibet.④Among different animals, the order of animal health risk was lamb>calf>dairy cow, and the order of mycotoxin risk in lamb was AFs>ZEN>DON>FB when fed with the polluted corn silage.In summary, the over-standard rates of mycotoxins in whole plant corn and whole plant corn silage in China were low.The relatively polluted areas were Huang-Huai-Hai, Central and Southwest China.In these areas, when silage corn was used as the main roughage for lactating dairy cows, the over-standard risk was higher;When corn silage was used as the main roughage for young animals, AFs contamination might bring animal health risks.

Key words: mycotoxins; silage corn; risk assessment

CLC Number:

S816.6

ZHANG Xinxin, ZHANG Fujin, ZHANG Yao, GUO Zhigang, SUN Lin, LIU Guanghua, SHA Na. Analysis of Mycotoxin Contamination and Animal Health Risk Assessment in Silage Corn of China[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4451-4459.

References

[1] 陈玲玲,玉柱,毛培胜,等.中国饲草产业发展概况及饲草料质量安全现状[J].饲料工业,2015,36(5):56-60. CHEN L L,YU Z,MAO P S,et al.Development situation of forage grass industry and current status of forage quality safety in China[J].Feed Industry,2015,36(5):56-60.(in Chinese)
[2] 张静.动物饲料与畜产品安全关系研究[J].畜牧兽医科学,2019,13:25-26. ZHANG J.Research on the relationship between animal feed and animal product safety[J].Graziery Veterinary Science,2019,13:25-26.(in Chinese)
[3] 武琳霞,李培武,丁小霞,等.农产品真菌毒素混合污染与累积风险评估研究进展[J].食品安全质量检测学报,2018,9(14):3553-3560. WU L X,LI P W,DING X X,et al.Advances in research on co-occurrence and cumulative risk assessment of mycotoxins in agricultural products[J].Journal of Food Safety and Quality,2018,9(14):3553-3560.(in Chinese)
[4] SLOBODCHIKOVA I,VUCKOVIC D.Liquid chromatography-high resolution mass spectrometry method for monitoring of 17 mycotoxins in human plasma for exposure studies[J].Journal of Chromatography A,2018,1548(4):51-63.
[5] MIMOUNE N A,ARROYO-MANZANARES N,GÁMIZ-GRACIA L,et al.Aspergillus section flavi and aflatoxins in dried figs and nuts in Algeria[J].Food Additives & Contaminants Part B-Surveillance,2018,11(2):119-125.
[6] SCHÖNEBERG T,JENNY E,WETTSTEIN F E,et al.Occurrence of Fusarium species and mycotoxins in Swiss oats——Impact of cropping factors[J].European Journal Agronomy,2018,92(1):123-132.
[7] SCHOEMAN A,FLETT B C,JANSEV B.Evaluating three commonly used growth media for assessing fumonisin analogues FB₁,FB₂ and FB₃ production by nine Fusarium verticillioides isolates[J].Food Additives & Contaminants Part A,2017,34(2):291-298.
[8] RASMUSSEN R R,STORM I M L D,RASMUSSEN P H,et al.Multi-mycotoxin analysis of maize silage by LC-MS/MS[J].Analytical and Bioanalytical Chemistry,2010,397(2):765-776.
[9] KAMALA A,ORTIZ J,KIMANYA M,et al.Multiple mycotoxin co-occurrence in maize grown in three agro-ecological zones of Tanzania[J].Food Control,2015,54(8):208-215.
[10] 龚阿琼,吴晓峰,陈法科,等.2017—2018年原料及饲料中霉菌毒素变化趋势[J].中国饲料,2019,7:89-92. GONG A Q,WU X F,CHEN F K,et al.The change trend of mycotoxin in raw materials and feed during 2017 to 2018[J].China Feed,2019,7:89-92.(in Chinese)
[11] 雷元培,周建川,王利通,等.2018年中国饲料原料及配合饲料中霉菌毒素污染调查报告[J].饲料工业,2020,41(10):60-64. LEI Y P,ZHOU J C,WANG L T,et al.A survey report on the mycotoxin contamination of Chinese raw materials and feed in 2018[J].Feed Industry,2020,41(10):60-64.(in Chinese)
[12] CODEX ALIMENTARIUS COMMISSION.CAC/GL 80-2013 Guidelines on the application of risk assessment for feed[S].Rome:Codex Alimentarius Commission,2013.
[13] DORNE J L,DOERGE D R,VANDENBROECK M,et al.Recent advances in the risk assessment of melamine and cyanuric acid in animal feed[J].Toxicology and Applied Pharmacology,2013,270:218-229.
[14] ROSSI C N,TAKABAYASHI C R,ONO M A,et al.Assessment of exposure of broiler chicken in Brazil to mycotoxins through naturally contaminated feed[J].Food Security,2013,5:541-550.
[15] COFFEY R,CUMMINS E.Feed to food risk assessment,with particular reference to mycotoxins in bovine feed[J].International Journal of Risk Assessment and Management,2008,8(3):266-286.
[16] 国家标准化管理委员会.GB/T 14699.1－2005 饲料采样[S].北京:中国标准出版社,2005. STANDARDIZATION ADMINISTRATION.GB/T 14699.1－2005 Feeding stuffs:Sampling[S].Beijing:Standards Press of China,2018.(in Chinese)
[17] 李鑫.多元生物毒素联合毒性统计分析[D].北京:北京林业大学,2016. LI X.Statistical analysis of multiple biotoxins’joint toxicity[D].Beijing:Beijing Forestry University,2016.(in Chinese)
[18] EUROPEAN FOOD SAFETY AUTHORITY.Scientific opinion on melamine in food and feed[J].EFSA Journal,2010,8(4):1573.
[19] 国家标准化管理委员会.GB 13078－2017 饲料卫生标准[S].北京:中国标准出版社,2018. STANDARDIZATION ADMINISTRATION.GB 13078－2017 Feed hygiene standard[S].Beijing:Standards Press of China,2018.(in Chinese)
[20] CODEX ALIMENTARIUS COMMISSION.CXS 193－2019 General standard for contaminants and toxins in food and feed[S].Rome:Codex Alimentarius Commission,2019.
[21] FOOD AND DRUG ADMINISTRATION.7371.004 Compliance program guidance manual program[S].USA,2004.
[22] EUROPEAN COMMISSION.EC No.1881/2006 Setting maximum levels for certain contaminants in foodstuffs[S].EU:Official Journal of the European Union,2006.
[23] JOINT FOOD AND AGRICULTURAL ORGANIZATION/WORLD HEALTH ORGANIZATION(FAO/WHO) EXPERT COMMITTEE ON FOOD ADDITIVES (JECFA).Evaluation of certain contaminants in food[A].Seventy-second Report of the Joint FAO/WHO Expert Committee on Food Additives[C].2011.
[24] JOINT FOOD AND AGRICULTURAL ORGANIZATION/WORLD HEALTH ORGANIZATION(FAO/WHO) EXPERT COMMITTEE ON FOOD ADDITIVES (JECFA).Evaluation of certain contaminants in food[A].Fifty-third Report of the Joint FAO/WHO Expert Committee on Food Additives[C].2000.
[25] JOINT FOOD AND AGRICULTURAL ORGANIZATION/WORLD HEALTH ORGANIZATION(FAO/WHO) EXPERT COMMITTEE ON FOOD ADDITIVES (JECFA).Evaluation of certain contaminants in food[A].Eighty-third Report of the Joint FAO/WHO Expert Committee on Food Additives[C].2017.
[26] 王国强,刘耀东,段胜,等.2019年上半年饲料及饲料原料霉菌毒素污染及调查报告[J].养猪,2019,6:17-20. WANG G Q,LIU Y D,D S,et al.Mycotoxin contamination of feed and feed materials in the first half of 2019 and investigation report[J].Swine Production,2019,6:17-20.(in Chinese)
[27] ABIA W A,WARTH B,SULYOK M,et al.Determination of multi-mycotoxin occurrence in cereals,nuts and their products in Cameroon by liquid chromatography tandem mass spectrometry (LC-MS/MS)[J].Food Control,2013,31(2):438-453.
[28] MAGNOLI C E,CAVAGLIERI L.Mycotoxigenic Fungi and Mycotoxins in Animal Feed in South American Countries[M].Cordoba:Naturales Universidad Nacional de Rio Cuarto,2015.
[29] 席俊程,殷术鑫,王璐,等.2018年不同地区全株玉米青贮品质分析[J].黑龙江八一农垦大学学报,2019,31(5):55-59. XI J C,YIN S X,WANG L,et al.Quality analysis of whole plant corn silage in different regions in 2018[J].Journal of Heilongjiang Bayi Agricultural University,2019,31(5):55-59.(in Chinese)
[30] 王文珺,桑华春.真菌毒素免疫检测技术的研究进展[J].食品安全导刊,2017,16:24-26. WANG W J,SANG H C.Research progress of mycotoxins immunoassay technology[J].China Food Safety Magazine,2017,16:24-26.(in Chinese)
[31] 兰静,赵琳,孙向东.中国玉米生物毒素污染现状及预防措施[J].安徽农业科学,2019,47(19):183-185. LAN J,ZHAO L,SUN X D,et al.Current situation and preventive measures of mycotoxin contamination of maize in China[J].Journal of Anhui Agricultural Science,2019,47(19):183-185.(in Chinese)
[32] WAGACHA J M,MUTHOMI J W.Mycotoxin problem in Africa:Current status,implications to food safety and health and possible management strategies[J].International Journal of Food Microbiology,2008,124(1):1-12.
[33] PAPST C,UTZ H F,MELCHINGER A E,et al.Mycotoxins produced by Fusarium spp.in isogenic Bt vs.non-Bt maize hybrids under European cornborer pressure[J].Agronomy Journal,2005,97(1):219-224.
[34] 郝渊鹏,李静一,杨瑞,等.芳香植物精油的抗菌性及在动物生产中的应用[J].植物学报,2020,55 (5):644-657. HAO Y P,LI J Y,YANG R,et al.Antimicrobial activity of aromatic plant essential oils and their application in animal production[J].Chinese Bulletion of Botany,2020,55(5):644-657.(in Chinese)
[35] EUROPEAN FOOD SAFETY AUTHORITY.Risks for animal health related to the presence of zearalenone and its modified forms in feed[J].EFSA Journal,2017,15(7):48-51.
[36] FOOD AND DRUG ADMINISTRATION.7371.003 Compliance program guidance manual program[S].Washington,D.C.:Food and Drug Administration,2010.
[37] ZIMMER I,USLENER E,WEBER R,et al.Fumonisin intake of the German consumer[J].Mycotoxin Research,2008,24(1):40-52.
[38] EUROPEAN FOOD SAFETY AUTHORITY.European food safety authority:Opinion of the scientific panel on contaminants in the food chain on a request from the commission related to aflatoxin B₁ as undesirable substances in animal feed[J].EFSA Journal,2004,39:1-27.

Analysis of Mycotoxin Contamination and Animal Health Risk Assessment in Silage Corn of China

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics

Comments

[1]	HE Mengru, ZHOU Ni, LYU Xiaohua. Risk Assessment of Bacitracin Resistance as a Medicated Feed Additive [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3749-3756.
[2]	HUANG Ke, WEN Xiaolu, WANG Li, JIANG Zongyong. Progress Research on the Hazard of Mycotoxins to Livestock and Poultry and the Methods of Reduction [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2600-2607.
[3]	ZHANG Liyun, WANG Yanxin, WANG Yuke, OU Yahong, PENG Dapeng, WANG Xu, WANG Yulian, PAN Yuanhu, XIE Shuyu, CHEN Dongmei, HUANG Lingli, TAO Yanfei. Research Progress on Electrochemical Biosensors in Detection of Mycotoxins [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2769-2777.
[4]	LIU Ning, YANG Zai-bin, YANG Wei-ren, JIANG Shu-zhen, ZHANG Gui-guo, YANG Ling, NIU Qun-sheng. Effects of Naturally Contaminated Corn and Corn Gluten Meal on Growth Performance, Apparent Digestibility and Serum Enzymes of Piglets [J]. , 2015, 42(3): 583-588.
[5]	FANG Si-min，BAI Fan，BAI Xue，GUO Chun-hua，ZHANG Zheng-fan，PENG Zhong-li. Research Progress on Detection Method，Hazard and Residual of Mycotoxins in Laying Hens [J]. , 2014, 41(6): 103-107.
[6]	DONG Shuang, HE Jian-bin, GUAN Xin, LONG Miao. Application of Flow Cytometry to Study Mycotoxins [J]. , 2014, 41(12): 258-262.
[7]	XU Zhen-wei, HAN Yi-yi, ZHENG Xiao-ping, HUANG Shi-xin, MENG Jin, GU Xin, LOU Fei, LI Jie, GAO Lei, ZHU Hong. Risk Assessment of Sodium Thiocyanate and Colony Count in Raw Milk [J]. , 2013, 40(S1): 18-22.
[8]	XIE Xiao-peng, YI Wei, ZHUANG Zhi-ming, ZHENG Jiao-que, ZOU Liu, XU Xi, PENG Meng-hua. Mycotoxins in Feed and its Prevention Measures [J]. , 2013, (5): 101-106.
[9]	HAN Rong-wei;WANG Jia-qi;ZHENG Nan;XU Xiao-min;ZHEN Yun-peng;QU Xue-yin;ZHAO Lian-sheng. Main Risk Factor Analysis for Milk Quality and Safety Ⅲ.Veterinary Drug Residues [J]. , 2012, 39(4): 1-10.
[10]	ZHENG Nan;WANG Jia-qi;;HAN Rong-wei;XU Xiao-min;;ZHEN Yun-peng;;QU Xue-yin;;ZHAO Lian-sheng. Toxicity of Mycotoxins in Milk: A Review [J]. , 2012, 39(3): 10-13.
[11]	ZHENG Nan;WANG Jia-qi;;HAN Rong-wei;XU Xiao-min;;ZHEN Yun-peng;;QU Xue-yin;;ZHAO Lian-sheng. Main Risk Factor Analysis for Milk Quality and Safety Ⅱ.Mycotoxins [J]. , 2012, 39(3): 1-9.
[12]	YAN Si-gang;WEI Zheng-ji. Risk Assessment System and Methods for Pig Major Infectious Diseases [J]. , 2011, 38(6): 247-252.
[13]	YAO Wen-sheng;FAN Xue-zheng;WANG Qin;NING Yi-bao. Qualitative Risk Assessment of Classical Swine Fever Qutbreak in China [J]. , 2011, 38(12): 195-199.
[14]	HOU Ranran; ZHANG Minhong. Damage of Mycotoxins on Animals and Advances in Study on Methods of Revention [J]. , 2007, 34(1): 13-16.