1株鹅源类志贺邻单胞菌的分离鉴定及致病性研究

doi:10.16431/j.cnki.1671-7236.2022.09.041

Abstract

Abstract: 【Objective】 This study was aimed to find out the causes of morbidity and mortality of geese in a goose farm in Jiangsu province and reduce economic losses.【Method】 The dead geese were dissected and the pathogenic bacteria identified by bacterial isolation and purification, staining observation, biochemical test, MALDI-TOF MS identification and 16S rDNA determination.The characteristics of the isolated strains were explored by drug sensitivity test and mouse pathogenicity test.【Result】 A Gram-negative short rod-shaped bacterium was isolated from the liver of diseased geese.The results of biochemical tests showed that the strain could ferment glucose, maltose, citrate and urea.MALDI-TOF MS identification showed that the strain was Plesiomonas shigelloides.16S rDNA sequence analysis showed that the similarity between the isolated strain and Plesiomonas shigelloides was the highest, which was more than 98%.The results of drug sensitivity test showed that the isolated bacteria were sensitive to 10 kinds of antibiotics such as levofloxacin, meropenem, cefoxitin and gentamicin.The isolate was intermediary to tetracycline, cefepime, ceftazidime and cefazolin, and resistant to 6 kinds of antibiotics including azithromycin, compound sulfamethoxazole and ampicillin.The isolate was typical multidrug-resistant bacterium.The results of mouse pathogenicity test showed that the median lethal dose of the isolated bacteria to mice was 5.0×10^6.5 CFU.【Conclusion】 This experiment first reported the isolation of pathogenic Plesiomonas shigelloides from geese.Levofloxacin, meropenem, cefoxitin and other effective commonly used clinical antibiotics were screened through drug sensitivity test.The isolate was highly pathogenic to mice, suggesting that the potential risks brought by it should not be ignored.

Key words: Plesiomonas shigelloides; geese; antibiotic susceptibility test; pathogenicity

CLC Number:

S852.61⁺1

KANG Yuhui, ZHOU Hongshan, JIN Wenjie, QIN Aijian. Isolation, Identification and Pathogenicity of a Strain of Plesiomonas shigelloides from Geese[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3676-3682.

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Isolation, Identification and Pathogenicity of a Strain of Plesiomonas shigelloides from Geese

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