河北省奶牛源大肠杆菌的分离鉴定、致病性及中草药体外抑菌效果研究

doi:10.16431/j.cnki.1671-7236.2022.09.042

Abstract

Abstract: 【Objective】 This study was aimed to analyze the pathogenicity of Escherichia coli(E.coli) from dairy cows in Hebei province and the antibacterial effect of Chinese herbal medicine in vitro.【Method】 30 feces samples with typical clinical symptoms were collected from dairy cows in Hebei province.The collected samples were cultured and observed, and the biochemical identification, serotype identification, transmission electron microscope observation, PCR amplification and pathogenicity study were carried out.9 kinds of Chinese herbal medicines were extracted by traditional decoction and alcohol extraction.The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by 96 well plate double dilution plate method.Chinese herbal medicines with strong antibacterial effect were selected and combined in two, the inhibition zone diameter(IZD) was measured by Oxford cup method, MIC and MBC were measured by 96 well plate double dilution plate method, and the antibacterial effect of the drug was evaluated.【Result】 12 strains of E.coli isolated from dairy cows were pathogenic, with a detection rate of 40%.The dominant serotype was O55, with a detection rate of 58.3%.In the liquid extract of Chinese herbal medicine, Phus chinensis and Schisandra chinensis had the strongest antibacterial effect, MIC and MBC were both 7.80 mg/mL, followed by Pructus mume, Prunella vulgaris and pomegranate peel, with a better antibacterial effect, MIC and MBC were both 15.63 mg/mL.In the alcohol extract of Chinese herbal medicine, Phus chinensis had the strongest antibacterial effect, MIC and MBC were both 3.97 mg/mL, followed by Schisandra chinensis, MIC and MBC were both 7.80 mg/mL.In the combination of two drugs, the combination of Schisandra chinensis and Pructus mume showed synergistic effect, and the antibacterial effect was the strongest, IZD was 26.00 mm, MIC and MBC were both 3.97 mg/mL.Secondly, the antibacterial effect of Phus chinensis and Pructus mume were better, IZD was 23.66 mm, MIC and MBC were both 7.80 mg/mL.【Conclusion】 The pathogenic Escherichia coli was successfully isolated in this study, and the dominant serotype was O55.Single and compound Chinese herbal medicines with good antibacterial effect were screened, which provided a scientific basis for the clinical application of traditional Chinese medicine in the prevention and treatment of cow diarrhea caused by Escherichia coli.

Key words: dairy cows; Escherichia coli; pathogenicity; Chinese herbal medicine; antibacterial effect

CLC Number:

S852.61⁺2

SHAO Mingzhu, ZHAO Yunqing, LI Songli, HOU Shaohua, ZHANG Yanying. Isolation and Identification of Escherichia coli from Dairy Cows in Hebei Province and Its Pathogenicity and Antibacterial Effect of Chinese Herbal Medicine in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3683-3691.

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Isolation and Identification of Escherichia coli from Dairy Cows in Hebei Province and Its Pathogenicity and Antibacterial Effect of Chinese Herbal Medicine in vitro

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