猪A群轮状病毒SCJY-13株的分离鉴定及致病性分析

doi:10.16431/j.cnki.1671-7236.2022.02.028

Abstract

Abstract: 【Objective】 This study was aimed to isolate and identify porcine Rotavirus group A (RVA) from feces of piglets suffered from diarrhea, and understand the pathogenicity of the isolate strain.【Method】 RVA was isolated and identified from piglet diarrhea feces by MA-104 cells, and orally infected healthy newborn piglets.The clinical symptoms were observed, the detoxification was detected by Real-time quantitative PCR, the pathological changes were observed by HE staining, and the distribution of virus was tested by immunohistochemistry (IHC).【Result】 A strain of G9P[23] RVA, named RVA/Pig-tc/CHN/SCJY-13/2017/G9P[23] (SCJY-13 strain for short), which could cause obvious cytopathy of MA-104 cells, was isolated, and the virus titer was 10^5.5 TCID₅₀/100 μL.Diarrhea occurred at 11 h post infection and lasted until 165 h post infection.The incidence rate was 100% (7/7), and the mortality rate was 28.57% (2/7).Viral nucleic acid could be detected from piglet anal swabs at 8 h post infection until 192 h post infection, and the peak appeared at 24 h post infection.At the same time, the viral load in each segment of small intestine reached the highest 54 h post infection, and the viral load in ileum was the highest, which was significantly higher than that in duodenum, jejunum and feces (P < 0.05).HE staining and IHC detection showed that SCJY-13 accumulated in villi and crypts of small intestine of infected piglets, especially in ileum.SCJY-13 strain infection caused a large number of lymphocyte infiltration in duodenum, jejunum and ileum villus lamina propria, mucosal epithelial cells and vacuolar degeneration at the tip of intestinal villus, increase of columnar cells, villus breaking and falling off, especially in ileum.【Conclusion】 The SCJY-13 strain could cause 100% morbidity of neonatal piglets and shed virus for a long time, and the virus was mainly distributed in the ileum of small intestine.These results provided a valuable reference for the pathogenicity study of procine RVA.

Key words: porcine; Rotavirus group A (RVA); G9P[23]; isolation and identification; pathogenicity

CLC Number:

S852.65⁺1

CHEN Xiaofei, ZHANG Bin, ZHANG Chunhong, ZHANG Jianfeng, LIAO Ming. Isolation, Identification and Pathogenicity Analysis of Porcine Rotavirus Group A Strain SCJY-13[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 660-668.

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Isolation, Identification and Pathogenicity Analysis of Porcine Rotavirus Group A Strain SCJY-13

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