鹅产蛋性状全基因组选择信号分析

doi:10.16431/j.cnki.1671-7236.2022.08.017

Abstract

Abstract: 【Objective】 The aim of this study was to screen candidate genes under selection associated with egg laying traits in Yili and Hortobágy geese using selection signal analysis.【Method】 Twenty-four adult Yili geese and Twenty-four adult Hortobágy geese with good health status and consistent feeding and management level were selected in this experiment.Blood samples were collected from the geese,genomic DNA was extracted,and whole genome resequencing technology was used for selection signal detection and analysis to select the candidate regions under selection,localized to the annotated genes,and then GO function and KEGG pathway enrichment analysis were performed to further screen the candidate genes related to laying traits in goose.【Result】 The average sequencing depth of whole genome resequencing was 15.28×,and the contrast rate with the reference genome was more than 97.31%.These two populations were analyzed by population differentiation index (Fst),and a total of 1 231 candidate regions were identified.After combined analysis with nucleotide diversity (Pi),a total of 10 candidate regions were selected from Yili geese population,and 5 annotated genes were obtained;A total of 353 candidate regions were selected from Hortobágy geese population,and 263 annotated genes were obtained.GO function and KEGG pathway enrichment analysis results showed that 6 candidate genes (BMP2,BMP6,MIS,ENO1,LIF,EP300) might be related to egg-laying traits in geese were initially selected,and IL-18 gene was also found to be possibly related to the avian immunity.【Conclusion】 The results of this study screened 6 candidate genes that might be associated with egg-laying traits in geese,and provided a bioinformatic reference for revealing the molecular regulatory mechanisms of egg-laying traits in geese.

Key words: Yili geese; Hortobágy geese; selection signal; egg-laying traits; candidate genes

CLC Number:

TANG Bihui, PAN Lu, LI Haiying, ZHAO Xiaoyu, WU Yingping, JIANG Teng, DING Yawen, WU Linan, CAO Yan, MEI Zhiyong. Genome-wide Selection Signal Analysis of Laying Traits in Geese[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3015-3025.

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Genome-wide Selection Signal Analysis of Laying Traits in Geese

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