中畜草原白羽肉鸭与樱桃谷鸭的遗传差异分析

doi:10.16431/j.cnki.1671-7236.2022.03.001

Abstract

Abstract: 【Objective】 This study was aimed to identify the genetic differentiation between IAS Grassland duck and Cherry Valley duck by genome resequencing technology, and investigate the genomic variation between the two breeds under different artificial selection to clarify the genetic basis for the formation of excellent traits.【Method】 In this study, 16 Cherry Valley ducks and 16 IAS Grassland ducks were adopted for genome resequencing.After filtering out SNP markers with high missing rates and low allele frequencies, high-quality SNPs were obtained for subsequent analysis.Then the genotypic data of the two breeds were analyzed by principal component analysis (PCA) to identify their genetic differentiation.The selected signals of IAS Grassland duck and Cherry Valley duck were comprehensively screened by population genetic differentiation index (Fst) and nucleic acid diversity ratio (Pi).【Result】 PCA analysis illustrated that IAS Grassland duck and Cherry Valley duck were significantly differentiated.The analysis values of Fst and Pi were calculated in 10 kb window and 5 kb step respectively, and the Top 1% of Fst and Pi values was taken as the threshold (Fst>0.177, Pi>0.885).A total of 410 kb candidate regions were screened in the signal overlap region of the two analysis methods, and a total of 21 candidate genes were annotated.GO and KEGG enrichment analysis showed that 12 candidate genes were enriched in two categories, including cellular component and biological process (P<0.05), and 2 genes were significantly enriched in the metabolic pathway, signal transduction, reproduction, immune response pathway, etc.(P<0.05).Among the significantly enriched genes, those related to lipid metabolism, amino acid metabolism, and immune modulation, including PDE3A, PRKAR2B, SEMA5A, SHANK2, STXBP6, and LOC101803508 (GOLGB1), were strongly selected.【Conclusion】 The results indicated that although both Cherry Valley ducks and IAS Grassland ducks were derived from Pekin ducks, they differentiated obviously after continuous artificial selection with different selection pressure and directions, IAS Grassland ducks had higher genetic diversity than Cherry Valley ducks.A series of candidate genes for genetic differentiation between the two duck breeds were predicted, and the relevant genes involved in the regulation of flavor and meat quality of White-feather meat ducks were mainly excavated, which provided a reference for the follow-up study of the characteristics of different White-feather meat ducks and the screening of variety specific molecular markers.

Key words: IAS Grassland duck; Cherry Valley duck; resequencing; selection signals; candidate genes

CLC Number:

S834.2

YU Simeng, GUO Zhanbao, HU Jian, ZHOU Zhengkui, HOU Shuisheng. Analysis of Genetic Differentiation Between IAS Grassland Ducks and Cherry Valley Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 801-808.

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Analysis of Genetic Differentiation Between IAS Grassland Ducks and Cherry Valley Ducks

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