Ⅰ群禽腺病毒血清11型的分离鉴定及致病性试验

doi:10.16431/j.cnki.1671-7236.2022.03.029

Abstract

Abstract: 【Objective】 This study was aimed to understand the genetic evolution and pathogenicity of Fowl adenovirus (FAdV) in Jiangsu area, and provide reference for the epidemiological study of FAdV and disease prevention and control.【Method】 Serological typing of the virus was determined by chick embryo passage culture, PCR identification, electron microscope observation, whole genome sequencing, sequence alignment and similarity analysis, and the isolated strain were injected into the chest muscle with a dose of 5×10^5.33 EID₅₀ to infect 10-day-old SPF chicks for animal regression experiments.【Result】 PCR results showed that the isolated strain was positive for group Ⅰ FAdV, and the spherical, membraneless, icosahedral structure of adenovirus was observed under transmission electron microscope.The whole genome and Hexon gene sequence analysis showed that the isolated strain was serotype 11 of FAdV D, named JSNT-1 strain.The mortality rate of SPF chicks infected with this strain was 10%(1/10).Autopsy of the dead chickens showed that the liver was discolored and yellow, bleeding and swelling, and the edge of liver was blunt and round, kidney was swollen, bleeding and pale.Detoxification could be detected in oral cavity and cloaca by Real-time PCR test, and the virus was distributed in many tissues and organs in the chicken body.The histopathological results showed that the liver cells of the dead chickens were degenerated and necrotic, with basophilic intranuclear inclusion bodies, glomerular epithelial cells were degenerated and necrotic.A large number of inflammatory cell infiltrations were seen in the myocardium.【Conclusion】 The isolated strain was serotype 11 of FAdV, and the clinical incidence was not obvious after infection with SPF chickens, and the fatality rate was low.The sick and dead chickens could produce characteristic inclusion body hepatitis lesions, and the virus could replicate in and outside the chickens.

Key words: FAdV-11; inclusion body hepatitis; isolation and identification; genetic evolution analysis; pathogenicity

CLC Number:

S852.65

LAN Hong, REN Guangcai, YE Junxian, XIONG Ting, HE Xianming, LIU Yufu, YANG Zekun, HAO Wenqian, CHEN Ruiai. Isolation, Identification and Pathogenicity of Fowl Adenovirus Group Ⅰ Serotype 11[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1067-1076.

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Isolation, Identification and Pathogenicity of Fowl Adenovirus Group Ⅰ Serotype 11

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