黄曲霉毒素B1、赭曲霉毒素A和玉米赤霉烯酮联合对奶山羊肠道微生物的影响

doi:10.16431/j.cnki.1671-7236.2017.12.004

Abstract

Abstract:

This experiment was conducted to investigate the effects of aflatoxin B₁ (AFB₁) individual,binary or tertiary mixed with ochratoxin A (OTA) and zearalenone (ZEN) on the bacterial composition and diversity of dairy goat intestinal tract. Twenty lactating Laoshan dairy goats with similar conditions were randomly assigned to five treatments (n=4). The dairy goats in control group (group A) were fed basal diet,while that of groups B,C,D and E were fed basal diet supplementation 50 μg/kg AFB₁+500 μg/kg ZEN,50 μg/kg AFB₁+100 μg/kg OTA,50 μg/kg AFB₁, 50 μg/kg AFB₁+100 μg/kg OTA+500 μg/kg ZEN,respectively. The experimental period consisted of 7 days for adaptation and 14 days for feeding mycotoxins. Faeces samples were collected on the 11th of training. The total DNA of feces was extracted using genomic DNA extraction kit,then 16S rRNA was amplified,and the 16S rRNA gene cloning library of dairy goat intestinal microorganism was constructed and data analysis was performed. The results of the dilution curves and the diversity index showed that single AFB₁ or mixed with other toxins had a tendency to reduce the dairy goat intestinal microorganism,but there was no significant difference (P>0.05).However,the total number of intestinal flora decreased significantly after AFB₁ mixed with other toxins treatement (P<0.05). The Firmicutes,Bacteroidetes and Tenericutes were found as the main phylum in the intestines of dairy goats using the species annotation of the obtained OTUs representative sequence. According to LEfSe analysis,the combination of AFB₁,OTA and ZEN could significantly increase the abundance of Succinivibrionaceae,Phascolarctobacterium and Aeromonadales (P<0.05).The abundance of Holdemania and BF311 bacillus in control group were significantly higher than those of other groups (P<0.05). These results indicated that AFB₁ mixed with ZEN and OTA could influence the gut microbial community structure of dairy goat,and the effects of different mycotoxin combinations complied with the following pattern:AFB₁+OTA+ZEN > AFB₁+OTA > AFB₁+ZEN > AFB₁.

Key words: dairy goat; mycotoxin; intestinal microflora; 16S rRNA

CLC Number:

S826

FAN Cai-yun, SU Di, LI Xiao-jiao, HUANG Shuai, ZHANG Yang-dong, ZHENG Nan, LIU Guang-lei, LIU Zhen, CHENG Jian-bo. Effects of Aflatoxin B₁ Individual,Binary or Tertiary Mixed with Ochratoxin A and Zearalenone on Intestinal Microorganism of Dairy Goat[J]. , 2017, 44(12): 3418-3425.

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Effects of Aflatoxin B₁ Individual,Binary or Tertiary Mixed with Ochratoxin A and Zearalenone on Intestinal Microorganism of Dairy Goat

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Effects of Aflatoxin B1 Individual,Binary or Tertiary Mixed with Ochratoxin A and Zearalenone on Intestinal Microorganism of Dairy Goat

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Effects of Aflatoxin B₁ Individual,Binary or Tertiary Mixed with Ochratoxin A and Zearalenone on Intestinal Microorganism of Dairy Goat