复合益生菌对肉鸡肠道微生物区系的影响

doi:10.16431/j.cnki.1671-7236.2022.02.010

Abstract

Abstract: 【Objective】 The aim of this study was to explore the effects of the compound probiotics composed of Lactobacillus casei, Lactobacillus acidophilus and Bifidobacterium lactis on the production performance and intestinal microflora of jute broilers, and provide theoretical basis for the application of compound probiotics in broiler production.【Method】 A total of 200 one-day-old male Jute broilers were randomly divided into 2 groups with 5 replicates per group and 20 broilers per replicate.Compound probiotics (Lactobacillus casei∶Lactobacillus acidophilus∶Bifidobacterium lactis=1∶1∶2) were added to the drinking water of broilers in probiotics group at a ratio of 1%.The broilers received normal drinking water acted as control group.At 42 days, the growth performance indexes were measured, and the intestinal microflora was analyzed by high-throughput sequencing.【Result】 Compared with control group, the average daily gain of broilers in probiotic group increased significantly (P < 0.05), the average daily feed intake, carcass yield and eviscerated carcass proportion increased, and F/G was reduced, but the differences were not significant (P > 0.05);The total OTU of the intestinal microflora, and Sob, Chao, Pd, Shannon, Shannoneven indexes of ileum in probiotic group were decreased, and the Simpson index increased, with no significant differences (P > 0.05).Firmicutes had the highest abundance in intestinal tract of broilers and were dominant.Compared with control group, no Lachnoclostridium was found in ileum of probiotic group.The abundance of Firmicutes and Lactococcus in ileum increased significantly or extremely significantly in probiotic group (P < 0.05 or P < 0.01), the abundance of Actinomycetes and Staphylococcus decreased significantly or extremely significantly (P < 0.05 or P < 0.01), and the abundance of Lacetospiraceae in cecum increased significantly (P < 0.05).【Conclusion】 Compound probiotics could improve the composition, function and diversity of intestinal microflora by increasing the abundance of Lactobacillus and reducing the abundance of pathogenic bacteria in ileum.At the same time, it could increase the abundance of Lachnospiraceae to improve the ability of intestinal to ferment and degrade non-starch polysaccharides, thereby improving feed utilization efficiency and promoting the growth of broilers.

Key words: probiotics; broilers; production performance; intestinal microflora

CLC Number:

S816.71

ZHANG Lihuan, JIA Hao, ZHANG Ruonan, WANG Yanfei, LIU Xuan. Effects of Compound Probiotics on the Intestinal Microflora of Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 488-500.

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Effects of Compound Probiotics on the Intestinal Microflora of Broilers

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