微生态制剂调控比格幼犬生长性能和肠道菌群的研究

doi:10.16431/j.cnki.1671-7236.2022.02.012

Abstract

Abstract: 【Objective】 This experiment was to explore the effects of compound probiotics on the growth performance and intestinal flora structure of Beagle puppies during lactation.【Method】 Twelve 15-day-old Beagle puppies were selected and randomly divided into control group and probiotics group, with 6 in each group.The puppies in the probiotics group were fed with 20 mL probiotic preparation daily, and the puppies in control group were fed with 20 mL normal saline.The body weight was measured and the weight gain was calculated on days 0, 3, 9, 12, 15, 18, 21, 25 and 28.Whole blood, serum and fecal samples were collected at the 28th day.Red blood cell count (RBC) and hemoglobin (HGB) concentrations were measured in whole blood samples, alkaline phosphatase (AKP), inorganic phosphorus (IP), total cholesterol (TCHO) content, triglyceride (TG), immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin-1β (IL-1β), lipopolysaccharide (LPS) contents were measured in serum samples.The fecal samples were sequenced for flora, the Goods coverage index, Observed species index, and Shannon index were counted.The samples were analyzed by PCoA, and the species distribution at the phylum, genus and species levels of each sample were counted.【Result】 Compared with control group, probiotics could significantly increase the weight of puppies (P < 0.05), and significantly increase the number of RBC and the content of HGB, AKP and IP in serum (P < 0.05), and promote the growth and development of Beagle puppies.Probiotics could significantly reduce serum TG content (P < 0.05), but had no significant effect on TCHO, IgA, IgG, IL-1β and LPS (P < 0.05).Observed species index and PCoA analysis results showed that the probiotics could significantly change the structure of puppies intestinal flora (P < 0.05).At the phylum level, the relative abundance of Bacteroides and Actinobacteriota in the puppies of the microecological preparation group was significantly lower (P < 0.05), the relative abundance of Fusobacteriota, Acidobacteria and Verrucomicrobia were significantly increased (P < 0.05).At the genus level, the relative abundance of Escherichia-Shigella, Holdemanella, Bifidobacterium and Blautia was significantly reduced (P < 0.05), the relative abundance of Clostridium sensu stricto 1, Streptococcus, Fusobacteria, Lactococcus and Pediococcus was significantly increased (P < 0.05).At the species level, the relative abundance of Escherichia coli and Bifidobacterium breve was significantly decreased (P < 0.05), the relative abundance of Clostridium colicanis, Lactococcus garvieae, Vagococcus teuberi, Bacillus velezensis, Enterococcus faecium and Fusobacterium mortiferum was significantly increased (P < 0.05).【Conclusion】 The probiotics for puppies could significantly promote the growth of puppies, increase their blood oxygen content, change the structure of the intestinal flora, and optimize the composition of the intestinal flora.

Key words: probiotics; Beagle puppies; growth performance; intestinal flora

CLC Number:

S829.2

WANG Zhihua, JIA Junpeng, RADEBE Stoffel Matjeke, YU Qinghua. Study on the Growth Performance and Intestinal Flora of Beagle Puppies Regulated by Probiotics[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 510-520.

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Study on the Growth Performance and Intestinal Flora of Beagle Puppies Regulated by Probiotics

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