中药联合益生菌对大肠杆菌致小鼠腹泻保护机制研究

doi:10.16431/j.cnki.1671-7236.2023.02.035

Abstract

Abstract: 【Objective】 Our previous study had shown that traditional Chinese medicine (TCM) combined with probiotics had obvious protective effect on diarrhea caused by pathogenic E.coli in mice.This study was aimed to further explore the protective mechanism of TCM combined with probiotics on diarrhea caused by pathogenic E.coli in mice.【Method】 Sixty mice were randomly divided into five groups: Blank group,model group,TCM group,probiotics group and TCM combined probiotics group.The diarrhea model of mice was established by intraperitoneal injection with 1×10⁷CFU/kg E.coli isolate suspension,TCM group was gavaged with TCM (2.5 g/kg dandelion extract,0.5 g/kg Astragalus total flavone and 0.5 g/kg Astragalus polysaccharide),and probiotics group was gavaged with Bacillus subtilis suspension (2×10⁸CFU/kg),TCM combined with probiotics group was gavaged with the corresponding dose of TCM and Bacillus subtilis (5×10⁷CFU/kg) mixture,once a day for 7 days.The pathological changes of small intestine tissue were observed by HE staining,the ultrastructural changes were observed by scanning electron microscope.The contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in intestine homogenate and serum of mice were determined by ELISA,the expression of TNF-α and IL-6 mRNA in intestine tissue and blood were determined by RT-PCR.The expression of nuclear factor-κB (NF-κB) p65 protein in small intestine was determined by immunohistochemical staining.【Result】 TCM combined with probiotics could improve the pathological and histological changes of small intestine,repair the phenomenon of high dehydration and atrophy of small intestinal villi and obvious shedding of microvilli.At the same time,TCM combined with probiotics could significantly reduce the contents of TNF-α and IL-6 in small intestine homogenate and serum (P＜0.05 or P＜0.01),significantly inhibit the expression of TNF-α and IL-6 mRNA in small intestine tissue and blood (P＜0.05 or P＜0.01).Furthermore,TCM combined with probiotics could significantly decrease the high expression of NF-κB p65 protein in small intestine.【Conclusion】 TCM combined with probiotics regulatesd NF-κB signaling pathway,inhibited the excessive expression of TNF-α and IL-6 mRNA,reduced the contents of TNF-α and IL-6 in intestine homogenate and blood,improved intestinal gland degeneration and mucosal inflammatory cell infiltration,as well as small intestinal villi and microvilli damage,thus playing a protective role against diarrhea caused by E.coli in mice.

Key words: E.coli; traditional Chinese medicine combined with probiotics; diarrhea; mice; mechanism

CLC Number:

S853.74

WANG Wei, LI Chunzhu, WANG Shasha, SANG Rui, GE Bingjie, LI Chunting, ZHAO Xin, LIU Xinman, YU Minghong, ZHANG Xuemei. Study on the Protective Mechanism of Traditional Chinese Medicine Combined with Probiotics on Diarrhea Caused by E.coli in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 779-788.

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Study on the Protective Mechanism of Traditional Chinese Medicine Combined with Probiotics on Diarrhea Caused by E.coli in Mice

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