槲皮素对PEDV感染幼龄仔猪的生长性能、血液生化指标和肠道吸收与屏障功能的影响

doi:10.16431/j.cnki.1671-7236.2022.09.015

Abstract

Abstract: 【Objective】 The study was to investigate the role of quercetin in the prevention and treatment of Porcine epidemic diarrhea virus (PEDV) infection in piglets.【Method】 Eighteen healthy weaned piglets (Ducoc×Landrace×Yorkshire, 7-day-old neonatal) with similar body weight were selected and randomly divided into 3 groups (control, PEDV and PEDV+ quercetin groups), with 6 replicates in each group and one piglet per replicate, the trial lasted for 11 d.After 3 d acclimation period, piglets in the PEDV+ quercetin group were orally administered with 10 mg/kg BW of quercetin during the days 4-10 of the experiment, the other groups of piglets were given the same volume of artificial milk.On the day 8 of the experiment, the piglets in PEDV and PEDV+ quercetin groups were orally administered with 1×10^4.5 TCID₅₀ PEDV, and the control group was given with the same volume of PBS.On the day 11 of the experiment, all piglets were weighed and orally administered with D-xylose (0.1 g/kg BW) on an empty stomach.One hour later, jugular vein blood samples were harvested to detect blood biochemical indexes, plasma diamine oxidase (DAO) activity and D-xylose content, and then all piglets were slaughtered and jejunal mucosa was taken to detect the relative expression of intestinal barrier function related genes, including villin, claudin-1, occludin and intestinal fatty acid binding protein (iFABP).【Result】 Compared with the control group, in PEDV group the average daily gain of piglets was significantly decreased (P<0.05), the fecal score was significantly increased (P<0.05);The content of HDL in blood was decreased significantly (P<0.05), and the contents of creatinine and urea nitrogen were increased significantly (P<0.05);The content of D-xylose in plasma was decreased significantly (P<0.05);The relative expressions of claudin-1 and iFABP genes in jejunum were significantly down-regulated(P<0.05).Compared with PEDV group, in PEDV +quercetin group the average daily gain of piglets and the content of D-xylose in plasma were significantly increased (P<0.05);The contents of creatinine and urea nitrogen in blood were significantly decreased (P<0.05);The relative expressions of claudin-1, villin and iFABP genes in jejunum were significantly up-regulated (P<0.05).【Conclusion】 10 mg/kg BW quercetin could alleviate the growth inhibition and renal function injury of piglets caused by PEDV infection to a certain extent, and improve the intestinal absorption and barrier function.

Key words: quercetin; Porcine epidemic diarrhea virus(PEDV); piglets; growth performance; blood biochemical indexes; intestinal absorption; intestinal barrier

CLC Number:

S816.7

WANG Manli, TAN Zihan, WANG Shuaijie, LI Qian, ZENG Yitong, WANG Lei, ZHAO Di, ZHANG Qian, HOU Yongqing. Effects of Quercetin on Growth Performance, Blood Biochemical Indexes and Intestinal Absorption and Barrier Function in Piglets Infected with PEDV[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3411-3418.

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Effects of Quercetin on Growth Performance, Blood Biochemical Indexes and Intestinal Absorption and Barrier Function in Piglets Infected with PEDV

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