奶山羊ACSL6基因克隆及表达调控分析

doi:10.16431/j.cnki.1671-7236.2021.07.003

Abstract

Abstract: The aim of this study was to obtain the coding sequences of long-chain acyl-CoA synthetase 6 (ACSL6) gene in Xinong Saanen dairy goats,and preliminarily explore its effect on lipid metabolism of mammary epithelial cells in dairy goats.Taking the primary mammary epithelial cells of Xinong Saanen dairy goats as experimental materials,ACSL6 gene was amplified and cloned,and tissue expression profiles were analyzed by Real-time quantitative PCR,bioinformatics analysis was carried out based on the sequence of ACSL6 gene by online software,RNA interference technology was applied to interfere the mRNA expression of ACSL6 gene in mammary epithelial cells of dairy goats.The results showed that the length of ACSL6 gene CDS region was 2 169 bp,encoded 722 amino acids.Bioinformatics analysis indicated that ACSL6 protein was an alkaline unstable protein.The results of tissue expression analysis showed that ACSL6 gene was highly expressed in mammary tissue,followed by spleen in dairy goats.The designed synthetic siRNA was transfected into mammary epithelial cells,interference of ACSL6 gene made the mRNA expression of acetyl-CoA carboxylase (ACC),stearyl-CoA dehydrogenase 1 (SCD1),cluster of differentiation 36 (CD36) and sterol regulatory element binding proteins 1 (SREBP1) genes extremely significantly decreased (P<0.01).This results laid the foundation for further study on the effect of ACSL6 gene on lipid metabolism at the protein level and individual level.

Key words: dairy goats; ACSL6 gene; cloning; bioinformatics analysis; RNA interference

CLC Number:

CHENG Fei, PAN Tan, ZHANG Fei, ZENG Xin, LUO Jun, LI Cong. Cloning and Expression Regulation Analysis of ACSL6 Gene in Dairy Goats[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2291-2301.

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Cloning and Expression Regulation Analysis of ACSL6 Gene in Dairy Goats

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