香猪3种组织环状RNA的表达及其功能分析

doi:10.16431/j.cnki.1671-7236.2020.12.009

Abstract

Abstract: The purpose of this study was to explore the expression and potential functions of circular RNA (circRNA) in the skin,subcutaneous fat,and longissimus dorsi muscle of Xiang pig.In this study,RNA-Seq technology and bioinformatics methods were used to analyze the expression of circRNAs in skin,subcutaneous fat,and longissimus dorsi muscle of Xiang pig,GO and KEGG enrichment analysis were performed for the host genes of three tissue-specific circRNAs,then target miRNA prediction was performed for the circRNAs that host genes associated with tissue structure and function.A total of 6 483 circRNAs were identified from the three tissues of Xiang pig,4 575,5 180 and 5 219 circRNAs were identified respectively from skin,subcutaneous fat and longissimus dorsi muscle respectively,among which 83,234 and 586 circRNAs expressed specifically.circRNAs distributed on chromosomes widely and mainly from exons,a few from introns and intergenic regions.The expression levels of most circRNAs were low.The host genes of skin-specific circRNAs were mainly involved inproteolysis,RNA transport,and gap junctions.Among them,host genes such as NF1 and MAPK1 were related to cutaneous diseases and might play a role in the form of circRNAs.The circRNA (circ-MAPK1) that the host gene MAPK1 could bind to miR-18a,miR-132,miR-411,and which might be involved in regulating the formation of collagen.The host genes of circRNAs expressed specifically in subcutaneous fat were enriched in adipocyte growth,development and cancer signaling pathways.ABHD5 and PTPN11 genes were related to fat metabolism,miR-103,miR-107 and miR-199a-5p bound by circ-PTPN11 could be involved in regulating adipocyte proliferation,differentiation and lipidosis.The host genes of circRNAs only expressed in longissimus dorsi muscle associated with cancer,infection,and muscle development.ROCK2 and PPP1CC were involved in myocyte differentiation and muscle contraction,while miR-339,miR-181a and miR-181b bound by circ-PPP1CC were involved in the regulation of myocyte differentiation,proliferation and growth.In conclusion,the circRNAs selected in this study and their combined miRNAs might be involved in skin collagen formation,fat deposition,and muscle cell differentiation and maturation.

Key words: Xiang pig; circular RNA (circRNA); skin; subcutaneous fat; longissimus dorsi muscle

CLC Number:

S828

ZHANG Xiao, RAN Xueqin, NIU Xi, HUANG Shihui, WANG Jiafu. Expression and Function Analysis of Circular RNA in Three Tissues of Xiang Pig[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3870-3881.

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Expression and Function Analysis of Circular RNA in Three Tissues of Xiang Pig

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