芒硝对驴皮成纤维细胞增殖、凋亡的影响

doi:10.16431/j.cnki.1671-7236.2020.07.021

Abstract

Abstract: The effect of different concentrations of mirabilite on the proliferation and apoptosis of donkey skin fibroblasts in vitro was evaluated in this study.The skin fibroblasts were cultured in vitro by the tissue block method,and identified by HE and Masson staining.Then,the fibroblasts were cultured with high (2 000,1 500 μg/mL),medium (1 000,500 and 250 μg/mL) and low concentration (100,50 and 10 μg/mL) of mirabilite.The proliferation rate and apoptosis rate of fibroblasts were measured by the methods of MTT and Caspase-3 respectively.Finally,the expression of genes and proteins that affected the collagen synthesis of donkey skin fibroblasts were detected by Real-time fluorescence quantitative PCR and ELISA in different concentrations of mirabilite media (1 500,250 and 10μg/mL).The results showed that the fibroblasts of donkey skin began to climb out from the edge of the tissue mass at the 5th day of culture,and grew up in the whole culture dish at the 25th day.HE staining showed a typical spindle shape,and the collagen fibers of donkey skin fibroblasts were blue by Masson staining.The proliferation rate of fibroblasts in the medium concentration group was significantly higher than that in the high and low concentration groups (P<0.05),correspondingly,apoptotic factor Caspase-3 activity was significantly lower in the medium concentration group (P<0.05).Compared with 24 h,the mirabilite could significantly increase the expression of genes related to collagen synthesis after 48 h (P<0.05),and the effect was best with 250 μg/mL of mirabilite.In summary,the method of tissue block culture was easy to isolate and culture donkey skin fibroblasts.250 μg/mL of mirabiliten could significantly improve the proliferation rate of donkey skin fibroblasts after 48 h and the expression of genes related to collagen synthesis.This result could provide theoretical basis for the study of the effects of mirabilite on the proliferation of donkey skin fibroblasts and the mechanism of wound healing.

Key words: donkey skin; fibroblast; mirabilite; collagen; genes

CLC Number:

S813.24

ZHANG Yuan, WANG Yanping, LIU Jiong, MIAO Kai, ZANG Rui, ZENG Weibin. Effect of Mirabiliteon on Proliferation and Apoptosis of Donkey Fibroblasts[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2159-2170.

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Effect of Mirabiliteon on Proliferation and Apoptosis of Donkey Fibroblasts

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