香猪卵巢circ_CSPP1的鉴定及功能预测分析

doi:10.16431/j.cnki.1671-7236.2021.11.015

Abstract

Abstract: The purpose of this study was to explore the potential functions of circ_CSPP1 in the ovarian tissues of Xiang pigs. In this study, the sequence of circ_CSPP1 was spliced by CIRI-full, the conservative analysis of circ_CSPP1 was calculated by online blat of circBase database, and the characteristics of circ_CSPP1 in the ovarian tissues of Xiang pigs were identified and analyzed by RT-PCR technology and Sanger sequencing technology. Moreover, miRanda, RNAhybrid and PITA softwares were used to predict and analyze the circ_CSPP1 bound miRNA molecular and miRNA target genes. Then GO and KEGG enrichment analyses were performed for the target genes. The results showed that the isolated circ_CSPP1 was conservative and originated from exons 8-12 of CSPP1 gene, which could be served as molecular sponges of 14 miRNAs, such as ssc-miR-324, ssc-miR-10a-5p, ssc-miR-9847-3p, ssc-miR-365-5p, ssc-miR-92b-5p, ssc-miR-885-3p, ssc-miR-7139-3p, ssc-miR-9851-3p, ssc-miR-33b-3p etc, and targeted 755 protein genes. GO enrichment analysis showed that the target genes of circ_CSPP1 were significantly enriched in 153 GO terms, in which cell maturation, cell division sites, negative regulation of cell migration, Wnt signaling pathway, cyclin binding, uterine development, activation of MAPK activity, focal adhesion and cleavage groove were related to ovarian function. KEGG enrichment analysis showed that the target genes of circ_CSPP1 were enriched into 260 signal pathways, of which 29 signal pathways were significantly enriched, and the target genes mainly concentrated in cancer pathway, cell cycle, estrogen signaling pathway, PI3K-AKT signaling pathway, ovarian steroidogenesis, oxytocin pathway and apoptosis etc. In conclusion, circ_CSPP1 might act as molecular sponges of many miRNAs to regulate the biological processes including proliferation and apoptosis of ovarian granulosa cells, follicle maturation, ovulation process, ovarian steroidogenesis and premature ovarian failure in the ovarian tissues of Xiang pigs.

Key words: Xiang pigs; circular RNA; circ_CSPP1; miRNA; ovaries

CLC Number:

S828

HUANG Yali, NIU Xi, LU Huan, HUANG Shihui, WANG Jiafu, RAN Xueqin, LI Sheng. Identification and Function Prediction Analysis of circ_CSPP1 in Ovarian Tissues of Xiang Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4055-4066.

References

[1] QU S, YANG X, LI X, et al. Circular RNA:A new star of noncoding RNAs[J]. Cancer Letters, 2015, 365(2):141-148.
[2] MEMCZAK S, JENS M, ELEFSINIOTI A, et al. Circular RNAs are a large class of animal RNAs with regulatory potency[J]. Nature, 2013, 495(7441):333-338.
[3] HANSEN T B, JENSEN T I, ClAUSEN B H, et al. Natural RNA circles function as efficient microRNA sponges[J]. Nature, 2013, 495(7441):384-388.
[4] DU W W, FANG L, YANG W, et al. Induction of tumor apoptosis through a circular RNA enhancing Foxo3 activity[J]. Cell Death and Differentiation, 2017, 24(2):357-370.
[5] LI Z, HUANG C, BAO C, et al. Exon-intron circular RNAs regulate transcription in the nucleus[J]. Nature Structural and Molecular Biology, 2015, 22:256-264.
[6] ZHANG Y, ZHANG X O, CHEN T, et al. Circular intronic long noncoding RNAs[J]. Molecular Cell, 2013, 51:792-806.
[7] XU H, GUO S, LI W, et al. The circular RNA Cdr1as, via miR-7 and its targets, regulates insulin transcription and secretion in islet cells[J]. Scientific Reports, 2015, 5:12453.
[8] LI F, ZHANG L, LI W, et al. Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/beta-catenin pathway[J]. Oncotarget, 2015, 6:6001-6013.
[9] ASHWAL FLUSS R, MEYER M, PAMUDURTI N R, et al. circRNA biogenesis competes with pre-mRNA splicing[J]. Molecular Cell, 2014, 56:55-66.
[10] DU W W, YANG W, LIU E, et al. Foxo3 circular RNA retards cell cycle progression via forming ternary complexes with p21 and CDK2[J]. Nucleic Acids Research, 2016, 44:2846-2858.
[11] ZHENG Q, BAO C, GUO W, et al. Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAs[J]. Nature Communications, 2016, 7:112-115.
[12] CHEN J, LI Y, ZHENG Q, et al. Circular RNA profile identifies circPVT1 as a proliferative factor and prognostic marker in gastric cancer[J]. Cancer Letters, 2017, 388:208-219.
[13] ZHU M, XU Y, CHEN Y, et al. Circular BANP, an upregulated circular RNA that modulates cell proliferation in colorectal cancer[J]. Biomedicine and Pharmacotherapy, 2017, 88:138-144.
[14] ZHAO Z, LI X, GAO C, et al. Peripheral blood circular RNA hsa_circ_0124644 can be used as a diagnostic biomarker of coronary artery disease[J]. Scientific Reports, 2017, 7:39918.
[15] LIANG G, YANG Y, NIU G, et al. Genome-wide profiling of Sus scrofa circular RNAs across nine organs and three developmental stages[J]. DNA Research, 2017, 24(5):523-535.
[16] HUANG M, SHEN Y, MAO H, et al. Circular RNA expression profiles in the porcine liver of two distinct phenotype pig breeds[J]. Asian-Australasian Journal of Animal Sciences, 2018, 31(6):812-819.
[17] LI A, HUANG W, ZHANG X, et al. Identification and characterization of circRNAs of two pig breeds as a new biomarker in metabolism-related diseases[J]. Cellular Physiology and Biochemistry, 2018, 47(6):2458-2470.
[18] VENO M T, HANSEN T B, VENO S T, et al. Spatio-temporal regulation of circular RNA expression during porcine embryonic brain development[J]. Genome Biology, 2015, 16(1):245.
[19] CHEN J, ZOU Q, LV D, et al. Comprehensive transcriptional profiling of porcine brain aging[J]. Gene, 2019, 693:1-9.
[20] 张博生, 马君峰.中国小型猪种——香猪[J]. 畜牧兽医杂志, 2015, 34(4):79. ZHANG B S, MA J F.Chinese miniature pig breed-Xiang pigs[J]. Journal of Animal Husbandry and Veterinary Medicine, 2015, 34(4):79.(in Chinese)
[21] LIANG G, YAN J, GUO J, et al. Identification of ovarian circular RNAs and differential expression analysis between Meishan and Large White pigs[J]. Animals, 2020, 10(7):1114.
[22] GUO T Y, HUANG L, YAO W, et al. The potential biological functions of circRNAs during the initiation of atresia in pig follicles[J]. Domestic Animal Endocrinology, 2020, 72:106401.
[23] ROSS J W, HALE B J, SEIBERT J T, et al. Physiological mechanisms through which heat stress compromises reproduction in pigs[J]. Molecular Reproduction and Developmentment, 2017, 84(9):934-945.
[24] SUN Q, YU R, WANG C, et al. Circular RNA circ-CSPP1 regulates CCNE2 to facilitate hepatocellular carcinoma cell growth via sponging miR-577[J]. Cancer Cell International, 2020, 29(20):202.
[25] YANG W, XIE T.Hsa_circ_CSPP1/miR-361-5p/ITGB1 regulates proliferation and migration of cervical cancer (CC) by modulating the PI3K-AKT signaling pathway[J]. Reproductive Sciences, 2020, 27(1):132-144.
[26] LI Q H, LIU Y, CHEN S, et al. circ-CSPP1 promotes proliferation, invasion and migration of ovarian cancer cells by acting as a miR-1236-3p sponge[J]. Biomedicine and Pharmacotherapy, 2019, 114:108832.
[27] XUE Y F, LI M, LI W, et al. Roles of circ-CSPP1 on the proliferation and metastasis of glioma cancer[J]. European Review for Medical and Pharmacological Sciences, 2020, 24(10):5519-5525.
[28] 张晓, 冉雪琴, 牛熙, 等.香猪3种组织环状RNA的表达及其功能分析[J]. 中国畜牧兽医, 2020, 47(12):86-97. ZHANG X, RAN X Q, NIU X, et al. Expression and function analysis of circular RNA in three tissues of Xiang pig[J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(12):86-97.(in Chinese)
[29] GLAZAR P, PAPAVASILEIOU P, RAJEWSKY N.circBase:A database for circular RNAs[J]. RNA, 2014, 20(11):1666-1670.
[30] ZHONG Y Y, WANG Z Q, SONG X J, et al. Proliferation of ovarian granulosa cells in polycystic ovarian syndrome is regulated by microRNA-24 by targeting wingless-type family member 2B (WNT2B)[J]. Medical Science Monitor, 2019, 25:4553-4559.
[31] GUO T Y, ZHANG J B, WANG Y, et al. circINHA resists granulosa cell apoptosis by up regulating CTGF as a ceRNA of miR-10a-5p in pig ovarian follicles[J]. BBA-Gene Regulatory Mechanisms, 2019, 1862(10):194420.
[32] WOOD J R, STRAUSS J F.Multiple signal transduction pathways regulate ovarian steroidogenesis[J]. Reviews in Endocrine and Metabolic Disorders, 2002, 3(1):33-46.
[33] HOLT J E, PYE V, BOON E, et al. The APC/C activator FZR1 is essential for meiotic prophase Ⅰ in mice[J]. Development, 2014, 141(6):1354-1365.
[34] XU S R, WEI P, YANG Q L, et al. Transcriptome analysis revealed key signaling networks regulating ovarian activities in the domestic yak[J]. Theriogenology, 2020, 15(147):50-56.
[35] DI F, LIU J S, LI S, et al. ATF4 contributes to ovulation via regulating COX2/PGE2 expression:A potential role of ATF4 in PCOS[J]. Frontiers in Endocrinology, 2018, 15(9):669.
[36] BRZERT M, KRANC W, CELICHOWSKI P, et al. Expression of genes involved in neurogenesis, and neuronal precursor cell proliferation and development:Novel pathways of human ovarian granulosa cell differentiation and transdifferentiation capability in vitro[J]. Molecular Medicine Reports, 2020, 21(4):1749-1760.
[37] MISHRA S R, THAKUR N, SOMAL A, et al. Expression and localization of fibroblast growth factor (FGF) family in buffalo ovarian follicle during different stages of development and modulatory role of FGF2 on steroidogenesis and survival of cultured buffalo granulosa cells[J]. Research in Veterinary Science, 2016, 108:98-111.
[38] CHAVES R N, HELENA T D M M, BURATINI J J, et al. The fibroblast growth factor family:Involvement in the regulation of folliculogenesis[J]. Reproduction Fertility & Development, 2012, 24(7):905-915.
[39] LIU L, YANG M, WANG N, et al. New insights of subfertility among transplanted women:Immunosuppressive drug FK506 leads to calcium leak and oocyte activation before fertilization[J]. Journal of Cellular Biochemistry, 2018, 119(3):2964-2977.
[40] BAKER S J, REDDY E P.CDK4:A key player in the cell cycle, development, and cancer[J]. Genes & Cancer, 2012, 3(11-12):658.
[41] ZHANG X, WEI R, SUN Y, et al. AKT3 is a pivotal molecule of CDH22 and GFRA1 signal pathways regulating selfnenewal in female germline stem cells[J]. Stem Cells, 2019, 37(8):1095-1107.
[42] BASINI G, BUSSOLATI S, BAIONI L, et al. Gelatinases (MMP2 and MMP9) in swine antral follicle[J]. Biofactors, 2011, 37(2):117-120.
[43] PIKE R, ORTIZ-ZAPATER E, LUMICISI B, et al. KIF22 coordinates CAR and EGFR dynamics to promote cancer cell proliferation[J]. Science Signaling, 2018, 11(515):eaaq1060.
[44] GONZALO S, KREIENKAMP R, ASKJAER P.Hutchinson-Gilford progeria syndrome:A premature agingdisease caused by LMNA gene mutations[J]. Ageing Research Reviews, 2017, 33:18-29.
[45] REHMAN A A U, GENG C Y, WANG L, et al. Doxorubicin induces ER calcium release via Src in rat ovarian follicles[J]. Toxicological Sciences, 2019, 168(1):171-178.
[46] CHATTERJEE O, PATIL K, SAHU A, et al. An overview of the oxytocin-oxytocin receptor signaling network[J]. Journal of Cell Communication & Signaling, 2016, 10(4):355-360.
[47] LIU K, ZHAO J, CHEN C, et al. Epistatic evidence for gender-dependant slow neurotransmission signalling in substance use disorders:PPP1R12B versus PPP1R1B[J]. EBioMedicine, 2020, 61:103066.

Identification and Function Prediction Analysis of circ_CSPP1 in Ovarian Tissues of Xiang Pigs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Yanxi, WANG Chen, LUO Yuan, ZHOU Yuancheng, XU Zhiwen, PENG Yuanyi, SONG Zhenhui, LIU Xiao. circRNA Expression Profile Analysis of PK-15 Cells Response to Seneca Virus A Infection [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 280-289.
[2]	HUANG Yueli, RAN Xueqin, NIU Xi, LI Sheng, HUANG Shihui, WANG Jiafu. Study on 264 bp Structural Variation of 3'-flanking Region of CHD3 Gene in Xiang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3026-3035.
[3]	WANG Dong, LIANG Jiayue, WANG Xiaobing, FENG Juan, ZHANG Ru, XIA Huiyan, XIAO Hongmei. Research Progress of Non-coding RNA in the Development of Hair Follicles of Cashmere Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1817-1827.
[4]	ZHANG Yuming, CHEN Qiaoguang, HOU Zhaofeng, LIU Dandan, TAO Jianping, XU Jinjun. Research Progress on Chicken miRNAs and Their Regulation Role After Pathogenic Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1908-1916.
[5]	WANG Yueli, SHAO Zhiran, YI Jihai, WANG Yong, WANG Zhen, CHEN Chuangfu. miR-145a-3p Regulates Brucella Induced Mouse Macrophage RAW264.7 Autophagy by Targeting ATG14 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1117-1125.
[6]	HUANG Xiaogang, HAN Beibei, LI Ju, ZHANG Shouquan. Advance on the Role of microRNA in Mammalian Gametogenesis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 624-630.
[7]	ZHAI Yaying, SHI Qiaoting, CHU Qiuxia, ZHU Xiaoting, HUA Liushuai, ZHANG Zijing, CHEN Fuying, QI Xinglei, WANG Eryao, LYU Shijie. Differential Expression Analysis of Plasma Exosomes microRNA Between Donor and Recipient Cattle Before Embryo Transfer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 641-649.
[8]	HOU Junjie, SHI Zhuoyan, LIU Xiaoping, JI Xiang, CHU Xiaoran, SONG Zhen, WEN Fengyun. Research Progress on microRNA Regulation of Intramuscular Fat Deposition in Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4318-4326.
[9]	LU Huan, NIU Xi, HUANG Yali, LI Sheng, RAN Xueqin, HUANG Shihui, WANG Jiafu. Screening of Candidate miRNA for Regulating Xiang Pig Reproduction [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3118-3127.
[10]	YU Shigang, WANG Gang, LIAO Juan, XIE Wei. Screening and Identification of the microRNAs Associated with Intramuscular Fat Deposition in Muchuan Black-bone Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2713-2726.
[11]	CHEN Fang, SUN Manxi, WANG Zhiyong, LIU Chang, HUANG Shihui, WANG Jiafu, RAN Xueqin, NIU Xi. Association Analysis of Structural Variation of ADCY3 and IGF1 Genes with Reproductive Traits in Xiang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2920-2927.
[12]	SUN Manxi, CHEN Fang, WANG Zhiyong, LIU Chang, HUANG Shihui, LI Sheng, NIU Xi, WANG Jiafu, RAN Xueqin. Polymorphisms of Two Structural Variant in MAPK Signaling Pathway and Its Association Analysis with Growth Traits in Xiang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2448-2456.
[13]	MA Cheng, HU Wenping, SHI Tianpei, HOU Haobin, LI Haijing, ZHANG Xinhao, YANG Li, ZHANG Li. Identification and Analysis of miRNAs Expression in Skeletal Muscle of Wutou Donkey and Sanfen Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1894-1905.
[14]	LI Xuan, TONG Junshuo, ZHANG Dachong, WANG Bingyun, CHEN Shengfeng, LIU Canying. Research Advances on miRNA in Regulating the Apoptosis of Follicular Granulosa Cells and Its Mechanism [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4429-4441.
[15]	FENG Guanghang, JIANG Shengwei, LI Yaokun, LIU Guangbin, ZOU Xian, LIU Dewu. Research Advances on Regulation of Animal Follicular Development by miRNA [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4133-4142.