NEAT1在小鼠早期妊娠子宫组织中的表达和调节

doi:10.16431/j.cnki.1671-7236.2021.11.016

Abstract

Abstract: The test was aimed to study the expression and regulation of nuclear paraspeckle assembly transcript 1 (NEAT1) in uterine tissue during early pregnancy of mice, and provided scientific evidence for revealing the mechanism of NEAT1 in mouse embryo implantation. The models of early pregnancy, pseudopregnancy, artificial decidualization and steroid hormone treatments were constructed respectively, and the expression of NEAT1 in the uterine tissue was detected by RNAscope and Real-time quantitative PCR techniques. The results showed that the expression of NEAT1 was dynamically changing in uterine tissue in early pregnancy. NEAT1 was detected in endometrium and muscular layer on days 1-5 of pregnancy and mainly observed in endometrial luminal and glandular epithelial cells. NEAT1 was highly expressed in decidual cells on days 6-8 of pregnancy. There was no detectable NEAT1 staining at conceptus. Similar with those of early pregnancy, the expression of NEAT1 in pseudopregnant uteri was observed in endometrium and muscular layer. In the artificial decidulization model, NEAT1 was mainly localized in the decidualised cells and the expression level was extremely significant higher than that on the control group (P<0.01). In the steroid hormone treatment model, progesterone (P₄) and estradiol (E₂) could significantly up regulate the expression of NEAT1 (P<0.05), and had a more significant effect on the up regulation of NEAT1 expression in endometrial stromal cells. The above results indicated that NEAT1 was involved in regulating early pregnancy and decidulization processes in mice, and the expression level was mainly regulated by steroid hormones.

Key words: nuclear paraspeckle assembly transcript 1(NEAT1); early pregnancy; uterine tissue; mice

CLC Number:

S857.2

ZHANG Xinyan, JIA Yanni, CAI Rui, ZHANG Ruixue, JIN Yaping, LIN Pengfei. Expression and Regulation of NEAT1 in Mouse Uterine Tissue During Early Pregnancy[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4067-4073.

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Expression and Regulation of NEAT1 in Mouse Uterine Tissue During Early Pregnancy

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