外源性RFRP-3和MLT对雄鼠初情期生长及繁殖性能的影响

doi:10.16431/j.cnki.1671-7236.2022.07.020

Abstract

Abstract: 【Objective】 This experiment was conducted to investigate the effects of exogenous RF-amides related peptide 3 (RFRP-3) and melatonin (MLT) on the growth and reproductive performance of male Kunming mice at the beginning of estrus,and the relationship between them.【Method】 Male Kunming mice at the beginning of estrus were given normal saline,exogenous RFRP-3,MLT,and a mixture of RFRP-3 and MLT,respectively.The effects on average weight gain,average feed intake,feed gain ratio (F/G),testicular development,the secretion levels of serum growth hormone (GH) and luteinizing hormone (LH),and the relative expression of growth and reproduction-related genes in mice were examined.【Result】 Exogenous RFRP-3 inhibited the expression of GH gene,suppressed body weight gain,and improved F/G;While exogenous MLT promoted the expression of GH gene,resulting in faster body weight growth and lower F/G.Under the combined treatment of RFRP-3 and MLT,the expression of GH gene increased,and the body weight gain was similar to that of MLT group.RFRP-3 inhibited the expression of melatonin receptor 1A (Mtnr1A) and LH genes and the secretion of testosterone,and the number of spermatogonia at all levels and leydig cells in the testes of mice decreased;MLT promoted the expression of Mtnr1A and LH genes and the secretion of testosterone,and the leydig cell density extremely significantly increased (P＜0.01).The MLT and RFRP-3 cotreatment decreased the number of spermatogonia and leydig cells,and significantly decreased the secretion of testosterone (P＜0.05).【Conclusion】 Exogenous RFRP-3 inhibited the growth and reproductive performance of male mice at the puberty,while exogenous MLT promoted the growth and reproductive performance of male mice.

Key words: RFRP-3 gene; MLT gene; male mice; growth performance; reproductive performance

CLC Number:

S814.1

PAN Jianqiu, LIUFU Sui, WU Manli, JIANG Danli, OUYANG Hongjia, SHEN Xu, XU Danning, HE Jianhua, HUANG Yunmao. Effects of Exogenous MLT and RFRP-3 on Growth and Reproductive Performance of Male Mice at the Puberty[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2622-2630.

References

[1] TSUTSUI K,SAIGOH E,UKENA K,et al.A novel avian hypothalamic peptide inhibiting gonadotropin release[J]. Biochemical and Biophysical Research Communications,2000,275(2):661-667.
[2] HINUMA S,SHINTANI Y,FUKUSUMI S,et al.New neuropeptides containing carboxy-terminal RFamide and their receptor in mammals[J].Nature Cell Biology,2000,2(10):703-708.
[3] UBUKA T,MORGAN K,PAWSONA J,et al.Identification of human GnIH homologs,RFRP-1 and RFRP-3,and the cognate receptor,GRP147 in the human hypothalamic pituitary axis[J].PLoS One,2009,4(12):e8400.
[4] UKENA K,TSUTSUI K.Distribution of novel RFamide-related peptide-like immunoreactivity in the mouse central nervous system[J].Neuroscience Letters,2001,300(3):153-156.
[5] ELENI A,CLARISSE Q,LANCE J K,et al.Functional implications of RFRP-3 in the central control of daily and seasonal rhythms in reproduction[J].Frontiers in Endocrinology,2019,10:183.
[6] WANG X Y,GUO G L,ZHANG X,et al.Effect of RFRP-3,the mammalian or tholog of GnIH,on the epididymis of male rats[J].Theriogenology,2018,118:196-202.
[7] JOHNSON M A,FRALEY G S.Rat RFRP-3 alters hypothalamic GHRH expression and growth hormone secretion but does not affect Kiss-1 gene expression or the onset of puberty in male rats[J]. Neuroendocrinology,2008,88(4):305-315.
[8] JOHNSON M A,TSUTSUI K,FRALEY G S.Rat RFamide-related peptide-3 stimulates GH secretion,inhibits LH secretion,and has variable effects on sex behavior in the adult male rat[J].Hormones and Behavior,2007,51(1):171-180.
[9] HUO K,LI X,HU W A,et al.RFRP-3,the mammalian or tholog of GnIH,is a novel modulator involved in food intake and glucose homeostasis[J].Frontiers in Endocrinology (Lausanne),2020,11:194.
[10] KOVÁCS A,LÁSZLÓ K,GÁLOSI R,et al.Intraamygdaloid microinjection of RFamide-related peptide-3 decreases food intake in rats[J].Brain Research Bulletin,2014,107:61-68.
[11] AMARAL F G,CIPOLLA-NETO J.A brief review about melatonin,a pineal hormone[J].Archives of Endocrinology and Metabolism,2018,62(4):472-479.
[12] ROSANA F D,HELENE R,STEPHANIE D,et al.Melatonin potentiates the effects of metformin on glucose metabolism and food intake in high-fat-fed rats[J].Endocrinology,Diabetes & Metabolism,2018,1(4):e00039.
[13] 王梓颐,徐尚,任艳玲,等.褪黑素对绵羊生长性能、断奶应激及免疫蛋白的影响[J].中国畜牧杂志,2022,58(4):195-200. WANG Z Y,XU S,REN Y L,et al.Effects of MLT on growth performance,weaning stress and immune proteins in sheep[J].Chinese Journal of Animal Science,2022,58(4):195-200.(in Chinese)
[14] YU K,DENG S L,SUN T C,et al.Melatonin regulates the synthesis of steroid hormones on male reproduction:A review[J].Review Molecules,2018,23(2):447.
[15] QIN F J,ZHANG J,ZAN L S,et al.Inhibitory effect of melatonin on testosterone synthesis is mediated via GATA-4/SF-1 transcription factors[J].Reproductive Biomedicine Online,2015,31(5):638-646.
[16] WANG L,ZHUO Z Y,SHI W Q,et al.Melatonin promotes superovulation in sika deer (Cervus nippon)[J].International Journal of Molecular Sciences,2014,15(7):12107-12118.
[17] FORSBERG M,MADEJ A.Effects of melatonin implants on plasma concentrations of testosterone,thyroxine and prolactin in the male silver fox (Vulpes vulpes)[J].Journal of Reproduction and Fertility,1990,89(1):351-358.
[18] REKIK M,TABOUBI R,BEN S I,et al.Melatonin administration enhances the reproductive capacity of young rams under a southern Mediterranean environment[J].Animal Science Journal,2015,86(7):666-672.
[19] MURA M C,LURIDIANA S,PULINAS L,et al.Melatonin treatment and male replacement every week on the reproductive performance in Sarda sheep breed[J].Theriogenology,2019,135:80-84.
[20] REVEL F G,SABOUREAU M,PÉVET P,et al.RFamide-related peptide gene is a melatonin-driven photoperiodic gene[J].Endocrinology,2008,149(3):902-912.
[21] UBUKA T,INOUE K,FUKUDA Y,et al.Identification,expression,and physiological functions of Siberian hamster gonadotropin-inhibitory hormone[J].Endocrinology,2012,153(1):373-385.
[22] YUE L,QIN X J,LIU X F,et al.Melatonin receptor Mel1b and Mel1c mediated green light induced the secretion of growth hormone in anteri or pituitary of chicks[J].Photochemistry and Photobiology,2019,95(6):1387-1394.
[23] BAI X,CAO J,DONG Y,et al.Melatonin mediates monochromatic green light-induced satellite cell proliferation and muscle growth in chick embryo[J].PLoS One,2019,14(5):e0216392.
[24] HAN X H,HE Y Y,ZENG G L,et al.Intracerebroventricular injection of RFRP-3 delays puberty onset and stimulates growth hormone secretion in female rats[J].Reproductive Biology and Endocrinology,2017,15(1):35.
[25] IKA P S,ALEXANDRA R,JEREMY T S,et al.Effect of RF-amide-related peptide-3 on luteinizing hormone and follicle-stimulating hormone synthesis and secretion in ovine pituitary gonadotropes[J]. Endocrinology,2009,150(12):5549-5556.
[26] MOHAMMED A Z,HAYTHAM A,MOHAMMED A S,et al.The salutary action of melatonin and betaine,given singly or concomitantly,on cisplatin-induced nephrotoxicity in mice[J].Naunyn-Schmiedeberg's Archives of Pharmacology,2021,394(8):1693-1701.
[27] SOLEIMANI M M,AZIZI M,SADEGHZADEH F.The effect of melatonin on testis histological changes and spermatogenesis indexes in mice following treatment with dexamethasone[J].Drug and Chemical Toxicology,2020,8:1-10.
[28] ZHANG P F,ZHENG Y,LV Y H,et al.Melatonin protects the mouse testis against heat-induced damage[J].Molecular Human Reproduction,2020,26(2):65-79.
[29] ALVES E R,FERREIRA C G M,SILVA M V,et al.Protective action of melatonin on diabetic rat testis at cellular,hormonal and immunohistochemical levels[J].Acta Histochem,2020,122:151559.
[30] SUMIT S,KAZUYOSHI T,CHANDRA M C.Temporal phase relation of circadian neural oscillations alters RFamide-related peptide-3 and testicular function in the mouse[J].Neuroendocrinology,2010,91(2):189-199.
[31] HU K L,CHANG H M,LI R,et al.Regulation of LH secretion by RFRP-3--From the hypothalamus to the pituitary[J].Frontiers in Neuroendocrinology,2019,52:12-21.
[32] JO B H,CAROLINE A,JENS D M,et al.Roles of RFRP-3 in the daily and seasonal regulation of reproductive activity in female syrian hamsters[J].Endocrinology,2017,158(3):652-663.
[33] DUCRET E,ANDERSON G M,HERBISON A E.RFamide-related peptide-3,a mammalian gonadotropin-inhibitory ortholog,regulates gonadoropin-releasing hormone neuron firing in the mouse[J]. Endocrinology,2009,150(6):2799-2804.
[34] GONZALEZ-ARTO M,HAMILTON T R,GALLEGO M,et al.Evidence of melatonin synthesis in the ram reproductive tract[J]. Andrology,2016,4(1):163-171.
[35] FRUNGIERI M B,CALANDRA R S,ROSSI S P.Local actions of melatonin in somatic cells of the testis[J].International Journal of Molecular Sciences,2017,18(6):1170.
[36] KATARZYNA K,DANIEL B,MARIA R,et al.Melatonin concentration in peripheral blood and melatonin receptors (MT1 and MT2) in the testis and epididymis of male roe deer during active spermatogenesis[J].Theriogenology,2020,149:25-37.
[37] WISSAM H K,ELARA N M,JEAN-MARIE E,et al.Immunolocalization of RFamide-related peptide 3 in a desert rodent Gerbillus tarabuli during seminiferous epithelium cycle[J].Tissue Cell,2018,55:1-12.

Effects of Exogenous MLT and RFRP-3 on Growth and Reproductive Performance of Male Mice at the Puberty

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