MAPK信号通路2个结构变异多态性及其与香猪生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.07.020

Abstract

Abstract: The aim of this study was to investigate the genetic diversity of MAPK10 and MEF2C gene structural variants in mitogen-activated protein kinase (MAPK) signaling pathway and its association with growth traits in Xiang pigs,in order to provide a theoretical basis for breeding improvement of Xiang pigs in Guizhou.The population genetic variation of two structure variation (SV) loci (MAPK10-I₈-sv273 and MEF2C-I₁-sv71) were investigated in 233 Xiang pigs by PCR technology,the growth curve of Xiang pigs was made by Origin software,the polymorphic information content (PIC),heterozygosity (He),homozygosity (Ho) and effective allele number (Ne) were calculated by online software,and the correlation between genotyping results of two SVs and growth traits of Xiang pigs was analyzed by SPSS 26.0 software.The results showed that two SVs were polymorphic in Xiang pig population,there were three genotypes of DD,ID and II,and two alleles of D and I.The allele frequency of D in MAPK10-I₈-sv273 was higher than I,and the genotype frequency of DD was the highest (0.65).The average daily gain,body height and growth rate of the individual with DD genotype were greater than that with II genotype,which indicated that D allele had the genetic effect to improve the growth performance in Xiang pigs.The allele frequency of I in MEF2C-I₁-sv71 was higher than D,and the genotype frequency of II was the highest (0.51).The average daily gain,chest circumference and growth rate of the individuals with II genotype were higher than that with DD genotype,which showed that I allele could improve the growth performance in Xiang pigs.In summary,MAPK10-I₈-sv273 and MEF2C-I₁-sv71 were related to the growth and development of Xiang pigs,and could be used as candidate molecular markers for the selection of growth traits.

Key words: Xiang pigs; MAPK signaling pathway; structural variation; growth traits; association analysis

CLC Number:

S813.3

SUN Manxi, CHEN Fang, WANG Zhiyong, LIU Chang, HUANG Shihui, LI Sheng, NIU Xi, WANG Jiafu, RAN Xueqin. Polymorphisms of Two Structural Variant in MAPK Signaling Pathway and Its Association Analysis with Growth Traits in Xiang Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2448-2456.

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Polymorphisms of Two Structural Variant in MAPK Signaling Pathway and Its Association Analysis with Growth Traits in Xiang Pigs

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