绵羊FUT8基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.10.016

Abstract

Abstract: 【Objective】 This study was aimed to explore the polymorphism of fucosyltransferase 8 (FUT8) gene g.74522417 C＞T and its effect on the growth traits in sheep,and screen the new genetic markers that could be used for molecular breading in sheep.【Method】 The g.74522417 C＞T of FUT8 gene in Hu sheep (n=992) and Ujumqin sheep (n=333) were typed by Sequenom MassARRAY? SNP typing technology,Illumina OvineSNP 600K BeadChip and Illumina OvineSNP50K BeadChip chip detection technology,and analyzed the association between g.74522417 C＞T and growth traits in Hu and Ujumqin sheep.【Result】 There were three genotypes (CC,TC and TT) of FUT8 gene g.74522417 C＞T in Hu and Ujumqin sheep,respectively.Population genetic analysis showed that g.74522417 C＞T was in moderate polymorphism in Hu and Ujumqin sheep (0.25＜PIC＜0.50).The result of χ²test showed that g.74522417 C＞T was in Hard-Weinberg equilibrium in two sheep population (P＞0.05).Association analysis revealed that FUT8 gene g.74522417 C＞T was significantly associated with body length at 4 months of age and body height at 6 months of age in Ujumqin sheep (P＜0.05),was significantly or extremely significantly associated with shin circumference at 3 months of age,waist angle width and shin circumference at 5 months of age,and loin muscle area and shin circumference at 6 months of age in Hu sheep (P＜0.05 or P＜0.01).【Conclusion】 FUT8 gene g.74522417 C＞T could be used as a potential molecular marker for growth and development in sheep,providing a reference for the application of FUT8 gene in molecular breeding of sheep.

Key words: sheep; FUT8 gene; polymorphisms; growth traits

CLC Number:

S813.3

FENG Mian, SHANG Mingyu, BI Yazhen, HU Wenping, ZHANG Li. Polymorphisms of FUT8 Gene and Its Association Analysis with Growth Traits in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3838-3845.

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Polymorphisms of FUT8 Gene and Its Association Analysis with Growth Traits in Sheep

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