China Animal Husbandry and Veterinary Medicine ›› 2021, Vol. 48 ›› Issue (8): 2920-2927.doi: 10.16431/j.cnki.1671-7236.2021.08.024

• Genetics and Breeding • Previous Articles     Next Articles

Association Analysis of Structural Variation of ADCY3 and IGF1 Genes with Reproductive Traits in Xiang Pigs

CHEN Fang1,2, SUN Manxi1,2, WANG Zhiyong1,2, LIU Chang3, HUANG Shihui4, WANG Jiafu1,2, RAN Xueqin1,4, NIU Xi1,2   

  1. 1. College of Life Sciences, Guizhou University, Guiyang 550025, China;
    2. Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China;
    3. Guizhou University of Traditional Chinese Medicine School of Pharmacy, Guiyang 550025, China;
    4. College of Animal Science, Guizhou University, Guiyang 550025, China
  • Received:2021-03-09 Online:2021-08-20 Published:2021-08-19

Abstract: The aim of this study was to investigate the genetic diversity of structural variation (SV) of adenylatecyclase 3 (ADCY3) and insulin like growth factor 1 (IGF1) genes in the ovarian steroidogenesis signaling pathway and their correlation with reproductive traits in Xiang pig sows.The SV of ADCY3 and IGF1 genes was studied in 269 Xiang pigs by PCR technique, the genetic heterozygosity (He), genetic purity(Ho), effective allele number(Ne) and polymorphic information content (PIC) were calculated using online software, and the association of different genotypes with reproductive traits such as total litter size, live litter size, newborn weight and number of teats in primiparous and multiparity Xiang pigs were analyzed using One-Way ANOVA and LSD method.The results showed that two SVs ADCY3-I1-sv506 and IGF1-I3-sv302 contained plenty of polymorphisms in Xiang pigs population, there were three genotypes of DD, DI and II, and two alleles of D and I.DI genotype (0.569) and D allele (0.601) were dominant in ADCY3-I1-sv506, II genotype (0.717) and I allele (0.816) were dominant in IGF1-I3-sv302.ADCY3-I1-sv506 and IGF1-I3-sv302 were moderate polymorphism (0.25<PIC<0.5), the heterozygosity was 0.479 and 0.300, respectively.The correlation analysis results showed that the total number born of primiparous and multiparous sows of DD genotype in ADCY3-I1-sv506 were significantly higher than that of II genotype (P<0.05), the total number born of primipara and multiparous sows and the number born alive of multiparous sows of II genotype in IGF1-I3-sv302 was significantly higher than that of DI genotype(P<0.05).In conclusion, both of two SVs in of ADCY3 and IGF1 genes had correlation ship with the reproductive performance of Xiang pigs, it could be taken as the candidate molecular markers for breeding of Xiang pigs sows.

Key words: Xiang pigs; ADCY3 gene; IGF1 gene; structural variation; reproductive traits

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