对香豆酸影响猪常温保存精液品质的研究

doi:10.16431/j.cnki.1671-7236.2021.08.025

Abstract

Abstract: The purpose of this study was to investigate the effects of different concentrations of P-coumaric acid (PCA) added to Beltsville thawing solution (BTS) on boar semen quality and antioxidant enzyme activities.The concentrations of 0, 0.03, 0.06, 0.09, 0.12, 0.15 g/L PCA were added into boar semen dilution preserved at room temperature.The sperm kinetic parameters and sperm functional integrity were detected by CASA automatic sperm analyzer and fluorescence probe technology within 1-5 d.The levels of reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by antioxidant kit on day 1, 3 and 5.The results showed that the sperm motility of the 0.09 g/L PCA group was significantly higher than that of the blank group on the 5th day of semen preservation (P<0.05), the sperm acrosome function integrity, plasma membrane function integrity and mitochondrial function integrity were also significantly higher than blank group (P<0.05).In addition, 0.03, 0.06, 0.09 and 0.12 g/L PCA treatment significantly reduced the levels of ROS and MDA, and increased the activity of SOD (P<0.05).The antioxidant activity of 0.09 g/L PCA, which in comparison with other treatment groups, was won the optimum antioxidant effect.However, when the adding concentration of PCA up to 0.15 g/L, the antioxidant activity of sperm was significantly decreased in comparison with 0.09 g/L (P<0.05).In summary, the addition of 0.09 g/L PCA to BTS could improve the quality of sperm, the best supplementation concentration of PCA was 0.09 g/L.

Key words: boar semen; PCA; room temperature preservation; sperm kinetic; functional integrity; antioxidant enzyme activity

CLC Number:

S814.1

LAN Qun, XIE Yingyu, CAO Jiacheng, XUE Li'e, LI Jiaquan, ZHAO Fanglu, LIN Ruiyi, FANG Shaoming, XIAO Tianfang. Effect of P-coumaric Acid on the Quality of Boar Semen Preserved at Room Temperature[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2928-2936.

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Effect of P-coumaric Acid on the Quality of Boar Semen Preserved at Room Temperature

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