版纳微型猪近交系精子冷冻保存研究

doi:10.16431/j.cnki.1671-7236.2017.01.023

Abstract

Abstract:

In this study, different concentrations of glycerol were added to semen cryoprotective extenders of Banna Mini-pig inbred line (BMI), different thawing solutions and thawing methods were studied. After thawing, the motility, the rates of plasma integrity and acrosome integrity were compared to optimize the cryopreservation method of BMI semen. The results showed that the motility, the rates of plasma integrity and acrosome integrity were significantly higher in 2% and 3% glycerol groups than those in 1%, 4% and 5% glycerol groups (P<0.05);The motility, the rates of plasma integrity and acrosome integrity were significantly higher in thawing solution Ⅰ than those in thawing solution Ⅱ, Ⅲ and Ⅳ (P<0.05); Through comparing the motility, the rates of plasma integrity and acrosome integrity, the thawing effect in 40℃ 6 s and 50℃ 6 s groups were better than that in 38℃ 30 s (P<0.05). In conclusion, the optimal semen cryoprotective method for BMI was 2% or 3% glycerol as cryoprotectant, using thawing solution Ⅰ to thaw at 40℃ 6 s or 50℃ 6 s.

Key words: BMI; boar semen; cryopreservation; thawing solution

CLC Number:

S814.8

ZHA Xing-qin, XIAO Jing, CHENG Wen-min, HUO Jin-long, WANG Shu-yan, WANG Pei, PAN Wei-rong, ZENG Yang-zhi. Study of Semen Cryopreservation for Banna Mini-pig Inbred Line[J]. , 2017, 44(1): 167-172.

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Study of Semen Cryopreservation for Banna Mini-pig Inbred Line

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