聚乙烯吡咯烷酮对公猪精液冷冻的影响

doi:10.16431/j.cnki.1671-7236.2020.08.024

Abstract

Abstract: The purpose of this study was to investigate the effect of polyvinylpyrrolidone (PVP) on boar semen freezing.There were five groups in the experiment:Control group (not add PVP) and PVP treatment groups (add 0.25%,0.50%,1.00%,and 2.00% PVP to the frozen base fluid).Collecting the semen of Songliao Black pig by hand,diluted with five frozen base solutions,equilibrated for 1 h at 25 ℃,2 h at 17 ℃,3 h at 4 ℃,then filled in 0.5 mL straw,fumigated 3 cm above liquid nitrogen for 10 min,stored in a liquid nitrogen tank for 30 d and tested.Sperm viability,plasma membrane integrity,acrosome integrity,mitochondrial activity,DNA integrity,catalase (CAT) activity,superoxide dismutase (SOD) activity,glutathione peroxidase (GSH-Px) activity,reactive oxygen species (ROS) level and malondialdehyde (MDA) level were tested after thawing.The results showed that compared with the control group,adding 0.50% PVP to the frozen base fluid could significantly improve the sperm viability,plasma membrane integrity,acrosome integrity,mitochondrial activity,CAT activity,SOD activity,GSH-Px activity (P<0.05);and significantly reduced the sperm ROS and MDA level (P<0.05).Adding PVP was beneficial to improve DNA integrity,but had no significant effect on DNA integrity compared with the control group (P>0.05).In conclusion,adding PVP to boar semen in the frozen base solution could improve the quality of sperm after freezing and thawing,the best concentration was 0.50%.

Key words: boar; semen; polyvinylpyrrolidone (PVP); cryopreservation; semen quality

CLC Number:

S823.8⁺3

JIAN Xinrui, QIAN Furong, WANG Jun, LI Xiaoxia, ZHANG Shaoxuan, ZHANG Zhibin, YU Yongsheng, GAO Kai, DIAO Yunfei, ZHANG Shumin, LI Zhaohua. Effect of Polyvinylpyrrolidone on Cryopreservation of Boar Semen[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2553-2560.

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Effect of Polyvinylpyrrolidone on Cryopreservation of Boar Semen

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